Zarkower D et al. (1994) Worm Breeder's Gazette "mab-3 YAC Rescue"

De Bono M, Hodgkin JA, Zarkower D

[Worm Breeder's Gazette, 1994]

mab-3 YAC rescue David Zarkower, Mario de Bono, and Jonathan Hodgkin MRC Laboratory of Molecular Biology, Cambridge, England

Nikolaou S et al. (2019) Dev Cell "Invasion by Force: The C.elegans Anchor Cell Leads the Way."

Nikolaou S, Machesky LM

[Dev Cell, 2019]

How cells breach basement membrane barriers remains an area of active research. In this issue of Developmental Cell, Kelley etal. (2019) reveal that the C.elegans anchor cell uses physical force to breach basement membrane in the absence of matrix metalloproteases during its developmental invasion.

Weinkove D (2018) BMC Biol "On microbes, aging and the worm: an interview with David Weinkove."

Weinkove D

[BMC Biol, 2018]

David Weinkove is an associate professor at Durham University, UK, studying host-microbe interactions in the model organism Caenorhabditis elegans. David has been focusing on the way microbes affect the physiology of their hosts, including the process of aging. In this interview, he discusses the questions shaping his research, how they evolved over the years, and his guiding principles for leading a lab.

Miller DM et al. (1992) Worm Breeder's Gazette "unc-4 LacZ Expression in A-Type Motor Neurons"

Miller DM, Niemeyer CJ

[Worm Breeder's Gazette, 1992]

unc-4 LacZ expression in A-type motor neurons David M. Miller and Charles J. Niemeyer, Dept. of Cell Biology, Duke Univ. Medical Ctr, Durham, NC 27710

Grenache DG et al. (1993) Worm Breeder's Gazette "Differential Effects of Dauer-Defective Mutations in L1-Specific Surface Antigen Switching"

Politz SM, Grenache DG

[Worm Breeder's Gazette, 1993]

DIFFERENTIAL EFFECTS OF DAUER-DEFECTIVE MUTATIONS ON L1- SPECIFIC SURFACE ANTIGEN SWITCHING. David G. Grenache and Samuel M. Politz, Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA.

Emmons SW et al. (1994) Worm Breeder's Gazette "Strain Names for non-C. elegans Species."

Emmons SW, Fitch DHA, Leroi AM

[Worm Breeder's Gazette, 1994]

Strain names for non-C. elegans species Scott W. Emmonst, Armand Leroit, and David Fitch, Department of Molecular Genetics, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461, Department of Biology, New York University, RmlOO9 Main Bldg., Washington Square, New York, NY 10003

Hall DH et al. (1994) Worm Breeder's Gazette "Cytology of Degenerin-Induced Cell Death in the PVM Neuron"

Driscoll MA, Chalfie M, Gu GQ, Hall DH, Gong L

[Worm Breeder's Gazette, 1994]

Cytology of degenerin-induced cell death in the PVM neuron David H. Hall, Guoqiang Gu+, Lei Gong#, Monica Driscoll#, and Martin Chalfie+, * Dept. Neuroscience, Albert Einstein College of Medicine, Bronx, N.Y. 10461 + Dept. Biological Sciences, Columbia University, New York, N.Y. 10027 # Dept. Molecular Biology and Biochemistry, Rutgers University, Piscataway, N.J. 08855

Carta LR et al. (1992) Worm Breeder's Gazette "Call for Worms"

Edgley ML, Thomas WK, Carta LR, Fitch DHA

[Worm Breeder's Gazette, 1992]

In response to the growing interest in worms related to C. elegans for comparative studies, we are coordinating an effort to collect a comprehensive array of cryopreservable species belonging to the order Rhabditida. This collection would be maintained at the Caenorhabditis Genetics Center, would be freely available to all interested scientists, and would provide an excellent resource for worm breeders who are interested in applying a broader phylogenetic viewpoint to comparative biological investigations. Of course, an important advantage of a universally accepted canonical set of living type species is that species identifications can be tested biologically through cross-mating experiments. The utility of such a collection has already been demonstrated in the Drosophila system a remarkable collection of species from around the world is maintained, for example, at the Bowling Green Stock Center in Ohio. We request that interested parties please send their wild isolates to David Fitch, Lynn Carta or Kelley Thomas, along with the following data: the date, source and method of isolation, any ecological information concerning the isolate, pertinent literature references, the names and addresses of the collector, the depositor and the taxonomist(1), and any specifics about stock maintenance. Other data about the species should also be included, such as measurements(2) and male tail characteristics. Scale illustrations and any anatomical, developmental, cytogenetic or molecular data are greatly appreciated. If the isolate is hermaphroditic, males should also be provided, since most of the morphological characters used in species identifications are associated with males. Males may occur spontaneously or can be induced by heat-shocking L4 or young adult hermaphrodites (usually, but not always, at 30 C for 6 hours). Males obtained in this way can be mated to hermaphrodites to maintain a stock containing males. Kelley will provide a molecular "identification tag". David and Lynn will determine if the species has been previously identified in the literature and serve as liaisons to nematologists with taxonomic expertise to help verify the species identification. We will then deposit the species with the CGC. Eventually, we hope to make all of the information associated with each species in the collection available in a database. (See the abstract by Fitch et al. in this issue for the latest information on current CGC species depositions). So hesitate not to share your pet species with us! We think that the effort in building a phylogenetically broad and comprehensive live collection of Rhabditida will be more than compensated by the valuable opportunities it will provide for developing novel approaches to many areas of nematode research.

Amrit FRG et al. (2017) J Vis Exp "Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project."

Ghazi A, Amrit FRG

[J Vis Exp, 2017]

Next generation sequencing (NGS) technologies have revolutionized the nature of biological investigation. Of these, RNA Sequencing (RNA-Seq) has emerged as a powerful tool for gene-expression analysis and transcriptome mapping. However, handling RNA-Seq datasets requires sophisticated computational expertise and poses inherent challenges for biology researchers. This bottleneck has been mitigated by the open access Galaxy project that allows users without bioinformatics skills to analyze RNA-Seq data, and the Database for Annotation, Visualization, and Integrated Discovery (DAVID), a Gene Ontology (GO) term analysis suite that helps derive biological meaning from large data sets. However, for first-time users and bioinformatics' amateurs, self-learning and familiarization with these platforms can be time-consuming and daunting. We describe a straightforward workflow that will help C. elegans researchers to isolate worm RNA, conduct an RNA-Seq experiment and analyze the data using Galaxy and DAVID platforms. This protocol provides stepwise instructions for using the various Galaxy modules for accessing raw NGS data, quality-control checks, alignment, and differential gene expression analysis, guiding the user with parameters at every step to generate a gene list that can be screened for enrichment of gene classes or biological processes using DAVID. Overall, we anticipate that this article will provide information to C. elegans researchers undertaking RNA-Seq experiments for the first time as well as frequent users running a small number of samples.

Vogel G (2002) Science "Neuroscience. Genes keep neurons' house in order."

Vogel G

[Science, 2002]

As any homeowner knows, timely maintenance is vital for keeping a building functioning properly after construction is finished. The same is evidently true for the complex architecture of the nervous system - at least in the roundworm. On page 686, neuroscientists Oliver Hobert, Oscar Aurelio, and David Hall describe a new family of proteins that help keep the wiring of the worm's nervous system tangle free.