Glater E, De Rosa MJ, Gourgou E, Deb A, Kamak M, Charlesworth AG, Sural S, Singhvi A, Pereira B, Akba K, Moyle MW, Ji EJ, Willis AR, Traa A, Giunti S, Soo S, Hernandez Lima M
[
J Neurogenet,
2020]
In the following pages, we share a collection of photos, drawings, and mixed-media creations, most of them especially made for this JoN issue, manifesting <i>C. elegans</i> researchers' affection for their model organism and the founders of the field. This is a celebration of our community's growth, flourish, spread, and bright future. Descriptions provided by the contributors, edited for space. <sup>1</sup>.
[
STAR Protoc,
2021]
Standard laboratory culture of <i>Caenorhabditis elegans</i> utilizes solid growth media with a bacterial food source. However, this culture method limits control of food availability and worm population density, factors that impact many life-history traits. Here, we describe liquid-culture protocols for precisely modulating bacterial food availability and population density, facilitating reliable production of arrested L1 larvae, dauer larvae, dietarily restricted worms, or well-fed worms. Worms can be grown in small quantities for standard assays or in the millions for other applications. For complete details on the use and execution of these protocols, please refer to Hibshman etal. (2016), Webster etal. (2018), and Jordan etal. (2019).