Zhao C et al. (1993) Worm Breeder's Gazette "Cloning of the lin-32 Gene"

Emmons SW, Zhao C

[Worm Breeder's Gazette, 1993]

Cloning of the lin-32 gene Connie Zhao and Scott W. Emmons, Department of Molecular Genetics, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461

Townsend SR et al. (1994) Worm Breeder's Gazette "C. elegans Molecular Genetics and Long PCR."

Finelli AL, Savage C, Xie T, Padgett RW, Townsend SR

[Worm Breeder's Gazette, 1994]

C. elegans Molecular Genetics and Long PCR Scott R. Townsend, Cathy Savage, Alyce L. Finelli, Ting Xie, and Richard W. Padgett, Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08855

Emmons SW et al. (1994) Worm Breeder's Gazette "Strain Names for non-C. elegans Species."

Emmons SW, Fitch DHA, Leroi AM

[Worm Breeder's Gazette, 1994]

Strain names for non-C. elegans species Scott W. Emmonst, Armand Leroit, and David Fitch, Department of Molecular Genetics, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461, Department of Biology, New York University, RmlOO9 Main Bldg., Washington Square, New York, NY 10003

Greenwald IS et al. (1983) Worm Breeder's Gazette "Tc1 polymorphisms on the right arm of LGIII."

Greenwald IS, Hodgkin JA

[Worm Breeder's Gazette, 1983]

As starting points for the molecular cloning of lin-12 III and tra-1 III, we have identified several Tc1 polymorphisms linked to these genes. Genomic DNA was prepared from appropriate Bristol/Bergerac hybrid strains, cut with EcoRI, and probed with Tc1 (kindly provided by Scott Emmons). Our data are summarized in the maps below, which give the approximate map locations and EcoRI fragment sizes of the Bergerac-specific Tc1's. [See Figure 1]

Lazaro-Pena, Maria I. et al. (2010) Worm Breeder's Gazette "The expression profile of innexins in the C. elegans male"

Emmons, Scott W., Lazaro-Pena, Maria I.

[Worm Breeder's Gazette, 2010]

Lewis JA (1981) Worm Breeder's Gazette "Modified Ficoll Method for Cleaning Worms"

Lewis JA

[Worm Breeder's Gazette, 1981]

I have made two modifications in the Ficoll method I originally described in WBG, vol. 3, #2. First, all Ficoll solutions are made 0. 1 M in NaCl to avoid shocking osmoticalIy sensitive worms like unc-29( e1072). Second, after sedimenting worms through 15% w/w Ficoll 400 ( 15', 300 x g), the worms are diluted with an equal volume of 0.1 M NaCl and floated on 35% w/w Ficoll (15', 300 x g) before washing 2x with 0.1 M NaCl. The Ficoll sedimentation removes dead or degenerated worms, cuticles, bacteria. The flotation removes crystalline debris sometimes occurring in worm cultures. We also find that special care to pipette out the last residue of bacterial medium (e.g. 3XD) before resuspending bacteria for worm growth is most conducive to obtaining uncontaminated worms.

Peterson KL et al. (1984) Worm Breeder's Gazette "isolation of new aldicarb resistant mutants in Bergerac."

Peterson KL, Russell RL

[Worm Breeder's Gazette, 1984]

We have isolated several Aldicarb resistant mutants in Bergerac using the heat shock mutagenesis protocol of Scott Emmons. We heat shocked 31,000 Bergerac hermaphrodites at 24 +/- 2 hours of age (at 16 C, timed from the middle of the egg laying period) at 28 C for 16 hrs. After this treatment, the animals were allowed to develop at 16 C and divided into 60 independent lines before the commencement of egglaying. Approximately 150,000 F1 hermaphrodites were collected, at maturity, these F1 were plated on 0.5mM Aldicarb and allowed to lay eggs until completion or death. The F2 progeny were screened 6 to 7 days later for the ability to grow in the presence of the drug. There appear to have been 40 independent mutational events that conferred Aldicarb resistance. All the strains isolated are uncoordinated to a greater or lesser degree. Three of these mutant strains showed a marked decrease (>10% w. t. levels) of choline acetyltransferase (ChAT) upon enzymatic assay and at least two of these strains have failed to complement cha-1; IV, the structural gene for ChAT. It is believed that if these new mutations are caused by transposition of Tc1 into cha-1 they could be valuable in the isolation and cloning of the gene.

Sommer RJ et al. (1994) Worm Breeder's Gazette "Evolution of Vulva-Formation: Part II: Species With a Central Vulva"

Sommer RJ, Sternberg PW

[Worm Breeder's Gazette, 1994]

Evolution of vulva-formation: Part II: Species with a central vulva Ralf J. Sommer & Paul W. Sternberg, California Institute of Technology, Division of Biology 156-29, Pasadena, CA 91125

Li WQ et al. (1992) Worm Breeder's Gazette "Characterization of the Axonal Guidance and Outgrowth gene Unc-33"

Shaw JE, Li WQ, Herman RK

[Worm Breeder's Gazette, 1992]

Characterization of the axonal guidance and outgrowth gene unc-33 W. Li, R. K. Herman and J. E. Shaw Department of Genetics and Cell biology, University of Minnesota, St Paul, MN 55108

Klacs M et al. (1982) Worm Breeder's Gazette "selection of spermatogenesis specific genes."

Klacs M, Herndon M

[Worm Breeder's Gazette, 1982]

We have recently begun a search for genomic DNA sequences that are transcriptionally active only during spermatogenesis by the following procedure: We have screened triplicate copies of a C. elegans clone bank (graciously provided by Scott Emmons) with three separate probes: 1) [32P]cDNA of Poly A RNA from synchronous L2 worms; 2) [32P]cDNA of Poly A RNA from 70% adult males; and 3) [32P]cDNA of Poly A RNA from gravid hermaphrodites. Plaques were screened and those plaques showing positive hybridization to only the male cDNA probe were picked for further analysis. So far of 1600 phage screened 200 were positive but only 2 of these showed specific hybridization only to the male probe. They have been picked as candidates for being spermatogenesis specific. Interestingly, three plaques were found to be hermaphrodite specific, hybridizing only to the gravid hermaphrodite probe. Our plan is to analyze these spermatogenesis specific sequences by determining their products and how they are regulated.