Bianchi L (2020) Neuron "C.elegans Glia Are Bona Fide Odorant Receptor Cells."

Bianchi L

[Neuron, 2020]

There is increasing evidence that supporting/glial cells in sensory systems function in sensory transduction. Duan etal. demonstrate that the glial cells of the C.elegans amphid apparatus serve as odorant receptor cells and regulate neuronal output and behavior.

Cheng H et al. (2022) STAR Protoc "Protocol for glial Ca2+ imaging in C.elegans following chemical, mechanical, or optogenetic stimulation."

Al-Sheikh U, Chen D, Duan D, Cheng H, Kang L

[STAR Protoc, 2022]

<i>Caenorhabditis elegans</i> is an exceptionally transparent model to analyze calcium (Ca²⁺) signals, but available protocols for neuronal Ca2⁺ imaging may not be suitable for studying glial cells. Here, we present a detailed protocol for glial Ca²⁺ imaging in <i>C.elegans</i> following three different approaches including chemical, mechanical, and optogenetic stimulation. We also provide the details for imaging analysis using Image-J. For complete details on the use and execution of this protocol, please refer to Duan etal. (2020).