Colonnello A et al. (2020) Neurotox Res "Correction to: Comparing the Neuroprotective Effects of Caffeic Acid in Rat Cortical Slices and Caenorhabditis elegans: Involvement of Nrf2 and SKN-1 Signaling Pathways."

Tunez I, Rubio-Lopez LC, Aguilera-Portillo G, Silva-Palacios A, Evaristo-Priego Y, Santamaria A, Galvan-Arzate S, Cortez-Nunez S, Aschner M, Rangel-Lopez E, Chen P, Colonnello A, Robles-Banuelos B, Garcia-Contreras R

[Neurotox Res, 2020]

One of the authors has incorrect family name spelling in the original article_. Michael Ashner should read as Michael Aschner.

Fodor A et al. (1983) Nematologica "Comparison of a new wild-type Caenorhabditis briggsae with laboratory strains of C. briggsae and C. elegans."

Fodor A, Nelson FK, Riddle DL, Golden JW

[Nematologica, 1983]

The hermaphrodite nematode strain G16, isolated from soil in Gujarat, India was identified as Caenorhabditis briggsae by genetic and morphological criteria. By contrast with the Dougherty strain of C. briggsae, in culture since 1944, the behavior of G16 resembles that of the wild-type C. elegans N2. The Gujarat population does not exhibit uncoordinated movement, it produces males which mate efficiently, it exhibits chemotaxis, and it forms dauer larvae in response to crowding or starvation. The G16 animals grow bigger, have a shorter generation time (1.6 days at 25C) and produce larger broods than the Dougherty strain. We conclude that the Dougherty strain has accumulated genetic defects during the years of laboratory cultivation. Genetic analysis using G16-Dougherty hybrids demonstrates that the defects in movements, chemotaxis, and dauer larva formation are all X-linked but genetically separable, while morphological differences in the male copulatory bursa are inherited autosomally. A Caenorhabditis-specific pheromone, which enhances entry into the dauer larva stage, is produced by the N2, G16, and Dougherty strains, but the Dougherty strain does not respond to the pheromone. The new wild-type C. briggsae may be more appropriate than the Dougherty strain for genetic study of C. briggsae or for future comparative

Dougherty EC et al. (1953) J Parasitol "The axenic cultivation of Rhabditis briggsae Dougherty and Nigon, 1949 (Nematosa: Rhabditidae). IV. Plasma protein fractions with various supplementation."

Keith DF, Dougherty EC

[J Parasitol, 1953]

The immediately preceding paper (Dougherty, 1953) has been confined to an account of work with liver as a source of factor Rb. In the present paper we deal with studies using human plasma as a source of this substance (or substances). A preliminary note briefly reporting a part of the results has already appeared (Dougherty, 1951). Whole plasma and its protein fractions and certain subfractions (See Haurowitz, 1950, pp. 148-160) have been tested. These studies throw additional light on the nature of factor Rb.

Silverman PH (1999) Science "C. elegans as a model."

Silverman PH

[Science, 1999]

Elizabeth Pennisi, in her excellent commentary "Worming secrets from the C. elegans" (News Focus, 11 Dec 1998, p.1972), states that "The first person to sense that the worm might take on such a prominent role in biology was molecular biologist Sydney Brenner." I am sure that Brenner would wish to acknowledge the role that Ellsworth C. Dougherty played in this matter. Dougherty originally described in 1949, "[a] new species of the free-living nematode genus Rhabditis of interest in comparative physiology and genetics".

Ferris H et al. (2015) Genetics "Ellsworth C. Dougherty: A Pioneer in the Selection of Caenorhabditis elegans as a Model Organism."

Ferris H, Hieb WF

[Genetics, 2015]

Ellsworth Dougherty (1921-1965) was a man of impressive intellectual dimensions and interests; in a relatively short career he contributed enormously as researcher and scholar to the biological knowledge base for selection of Caenorhabditis elegans as a model organism in neurobiology, genetics, and molecular biology. He helped guide the choice of strains that were eventually used, and, in particular, he developed the methodology and understanding for the nutrition and axenic culture of nematodes and other organisms. Dougherty insisted upon a concise terminology for culture techniques and coined descriptive neologisms that were justified by their linguistic roots. Among other contributions, he refined the classification system for the Protista.

Nicholas WL et al. (1962) Nematologica "The B-Vitamins required by Caenorhabditis briggsae (Rhabditidae)."

Hansen EL, Nicholas WL, Dougherty EC

[Nematologica, 1962]

To find which of the B vitamins are required by Caenorhabditis briggsae Dougherty & Nigon, it was cultured axenically in a known medium together with chick embryo extract. The concentration of each vitamin added to the medium was varied, and each was omitted in turn. Omitting thiamine, riboflavin, folic acid, calcium pantothenate, niacinamide and pyridoxine was deleterious.

Peel, Nina et al. (2011) International Worm Meeting "The SCFSEL-10 ubiquitin ligase complex is a key regulator of ZYG-1 levels."

Dougherty, Michael, O'Connell, Kevin, Peel, Nina

[International Worm Meeting, 2011]

The correct segregation of DNA during cell division requires formation of a bipolar spindle, organized at each pole by a centrosome. Regulation of centrosome duplication such that each mitotic cell has exactly two centrosomes is therefore of central importance to cell division. Deregulation of centrosome duplication causes the appearance of supernumerary centrosomes, which are a hallmark of many cancer cells and can contribute to tumorigenesis. Ectopic expression of the kinase Plk4, which is required for centrosome duplication causes the formation of extra centrosomes, moreover aberrant Plk-4 expression levels are associated with cancer. Data from Drosophila and human cells suggests that Plk4 levels are regulated by the SCFslimb/bTrcp ubiquibin ligase and proteosomal degradation. In C. elegans it is unclear how levels of the functional homolog of Plk4, ZYG-1, are controlled. We show that levels of ZYG-1 are regulated by proteosomal degradation as inhibition of proteosome function leads to an increase in ZYG-1 protein levels. We further show that RNAi-mediated down-regulation of SCF components SKR-1/2 causes an increase in ZYG-1 levels, indicating that this complex is required to target ZYG-1 for degradation. We do not however find a role for the slimb/bTrcp homolog lin-23 in ZYG-1 degradation. Surprisingly we find that the F-box protein SEL-10 is instead required to regulate ZYG-1 levels. Significantly, down-regulation of any of the SCFsel-10 components upregulates ZYG-1 activity sufficiently to restore centrosome duplication in a zyg-1 hypomorphic mutant. Moreover, we find that components of the SCF complex show an overlapping pattern of localization with ZYG-1, consistent with their regulating ZYG-1 levels. Our results show that precise control of ZYG-1 levels is achieved through its proteosomal degradation directed by the SCFSEL-10 complex.

Hengartner MO et al. (1992) Worm Breeder's Gazette "unc-50, a Gene Required for Acetylcholine Receptor Function, Encodes an Unfamiliar Protein"

Horvitz HR, Tsung N, Hengartner MO, Lewis JA

[Worm Breeder's Gazette, 1992]

unc-50, a gene requiired for acetylcholine receptor function, encodes an unfamiliar protein Michael Hengartner, Nancy Tsung, Jim Lewist, and Bob Horvitz HHMI, Dept. Biology, MIT, Cambridge, MA 02139, USA. t Division of Life Sciences, U. of Texas at San Antonio, San Antonio, IX 78249

Rothstein M et al. (1969) "Culture methods and nutrition of nematodes and Acanthocephala."

Nicholas WL, Rothstein M

[1969]

In order to study properly the nutrition and culture of nematodes, it is desirable to establish the organisms in axenic culture. Only in this way can the metabolic abilities of the nematodes be separated from those of coexisting and interacting organisms. One may settle for a mono-axenic culture, but the best way to attain this is to obtain axenic nematodes and then add the second organism or tissue, for example, alfalfa callus tissue for plant parasitic nematodes (Krusberg, 1961). This chapter will devote itself, in the main, to recent work on the culture and nutrition of nematodes, free-living and parasitic, and will refer only in passing to work already thoroughly reviewed (Dougherty et al., 1959; Nicholas, et al., 1959; Dougherty, 1960).

Holway AH et al. (2007) J Cell Biol "Checkpoint silencing during the DNA damage response in Caenorhabditis elegans embryos"

Kim SH, Michael WM, La Volpe A, Holway AH

[J Cell Biol, 2007]

After publication of this manuscript, the authors noticed that two images (Fig. 4 B, top left and middle) had been duplicated from a previous publication (Holway, A.H., C. Hung, and W.M. Michael. 2005. Genetics. 169:14511460). Here we provide new images corresponding to these control experiments. The authors apologize for this oversight.