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[
Worm Breeder's Gazette,
2002]
The purpose of this study was to investigate the effect of streptomycin sulphate in water solutions on the nematode life span. In this experiment streptomycin sulphate was used in following dilutions: 1.0 and 0.1 mg/ml. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without streptomycin sulphate) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without streptomycin sulphate in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3th day, these worms were transferred every day in next wells containing medium with streptomycin sulphate in any concentration. This investigation was carried out in temperature +21C and in the darkness.
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[
Worm Breeder's Gazette,
2003]
The purpose of this study was to investigate the effect of sodium salicylate in water solutions on the nematode life span. In this experiment sodium salicylate was used in following dilutions: 1000, 100, 10, 1.0 and 0.1 mg/L. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without sodium salicylate) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without sodium salicylate in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with sodium salicylate in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table
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[
Worm Breeder's Gazette,
2003]
The purpose of this study was to investigate the effect of acetylsalicylic acid in water solutions on the nematode life span. In this experiment acetylsalicylic acid was used in following dilutions: 1000, 100, 10, 1.0 and 0.1 mg/L. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without acetylsalicylic acid) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without acetylsalicylic acid in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with acetylsalicylic acid in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table
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[
Worm Breeder's Gazette,
2003]
The purpose of this study was to investigate the effect of glycerol in water solutions on the nematode life span. In this experiment glycerol was used in following dilutions: 100 g/L, 10 g/L, 1 g/L, 100 mg/L, 10 mg/L, 1 mg/L and 0.1 mg/L. Three adult animals (3-5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without glycerol) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without glycerol in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with glycerol in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table.
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[
Worm Breeder's Gazette,
2003]
The purpose of this study was to investigate the effect of kudesan (a water-soluble medicine, containing 30.0 mg of ubiquinone and 4.5 mg of a-tocopherol in 1 mL) in water solutions on the nematode life span. In this experiment kudesan was used in following dilutions: 1000, 100, 10, 1.0 and 0.1 mg/L. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without kudesan) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without kudesan in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with kudesan in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table.
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[
Worm Breeder's Gazette,
1996]
It is well known that the royal jelly markedly prolongs the life span of honey bees. This substance is recommended by physicians for increasing of life quality. In this experiment royal jelly extract was used in the form of "Apilac" in dilutions 0,02% and 0,002%. The medium for control group was prepared by mixing S medium containing E. coli with S medium without E. coli (1:1). Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0,75 ml of liquid medium (with E. coli and with or without royal jelly extract) during 6 hours, then they were discarded and newborn larvae were transferred in next wells every day (one worm in one well). Beginning from third day in the whole experiment was used the medium without this royal jelly extract. A number of progeny was calculated every day. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Control group Experimental Experimental group (0,02%) group (0,002%) n = 11 n = 11 n = 12 Mean +/- S.D. Mean +/- S.D. Mean +/- S.D Longevity (days): mean 19,4 +/- 1,2 18,0 +/- 1,7 21,8 +/- 1,2 maximal 25 27 26 minimal 10 9 14 Periods (days): prereproductive 2 2 2 reproductive 6,5 +/- 0,5 7,4 +/- 0,6 5,9 +/- 0,4 postreproductive 10,6 +/- 1,2 8,5 +/- 1,6 14,0 +/- 1,3 Fecundity: mean 158,8 +/- 6,6 93,9 +/- 9,9 128,9 +/- 3,4 maximal 197 179 153 minimal 115 60 111
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[
Worm Breeder's Gazette,
1995]
It is well known that low temperatures can prolong longevity of different animals. In this study the experimental worms were mantained in liquid medium with E. coli in +21 C during the day (8-20 hrs) and in +4 C during the night, in darkness. One control group was mantained in +21 C and other control group was mantained in +4 C constantly. The obtained results are presented in the following table. ......................................................................... Control group Experimental Control group (+21C) group (+4C) Mean +/- S.D. Mean +/- S.D. Mean +/- S.D. .......................................................................... Mean longevity (days) 19,86 +/- 1,63 22,96 +/- 1,57 38,30 +/- 2,72 (n = 22) (n = 24) (n = 22) Maximal longevity (days) 34 35 50 Minimal longevity (days) 6 10 5 Mean fecundity 76,91 +/- 4,54 54,33 +/- 3,32 4,45 +/- 2,07 (n = 22) (n = 24) (n = 22) Maximal fecundity 118 95 46 Minimal fecundity 33 25 0 .................................................................... It can be concluded that such intermittent temperature is not able to prolong the life-span of C. elegans significantly, in comparison with constant cold, as well as fecundity. Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (wild line) and E. coli OP50.
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[
Worm Breeder's Gazette,
2002]
The purpose of this study was to investigate the effect of acetylsalicylic acid in water solutions on the nematode life span. In this experiment acetylsalicylic acid was used in following dilutions: 1000, 100, 10, 1.0 and 0.1 mg/L. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without acetylsalicylic acid) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without acetylsalicylic acid in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with acetylsalicylic acid in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Conclusion: If acetylsalicylic acid solution was applied to C. elegans in concentration of 10 mg/L, it was able to increase significantly (P>0.05) their mean longevity in comparison with control to 83.96 percent.
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[
Worm Breeder's Gazette,
2003]
The purpose of this study was to investigate the effect of hydrogen peroxide in water solutions on the nematode life span. In this experiment hydrogen peroxide was used in following dilutions: 100 mg/L, 10 mg/L, 1 mg/L, 0.1 mg/L, 0.01 mg/L and 0.001 mg/L. Three adult animals (3-5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without hydrogen peroxide) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without hydrogen peroxide in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with hydrogen peroxide in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Conclusion: If hydrogen peroxide solution was applied to C. elegans, it was not able to increase their mean longevity significantly in comparison with control.
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[
Worm Breeder's Gazette,
1994]
Mutagenesis of C. elegans using N-ethyl-N-nitrosourea Elizabeth De Stasio, Dinesh Stanislaus and Catherine Lephoto. Department of Biology, Lawrence University, Appleton, Wl 54911