Vladimir V Bakaev (2002) Worm Breeder's Gazette "An attempt to slow aging in C. elegans. 26. A slight positive effect of streptomycin sulphate in concentrations 1.0 and 0.1 mg/ml"

Vladimir V Bakaev

[Worm Breeder's Gazette, 2002]

The purpose of this study was to investigate the effect of streptomycin sulphate in water solutions on the nematode life span. In this experiment streptomycin sulphate was used in following dilutions: 1.0 and 0.1 mg/ml. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without streptomycin sulphate) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without streptomycin sulphate in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3th day, these worms were transferred every day in next wells containing medium with streptomycin sulphate in any concentration. This investigation was carried out in temperature +21C and in the darkness.

Vladimir V Bakaev et al. (2003) Worm Breeder's Gazette "An attempt to slow aging in C. elegans. 29. No positive effect of sodium salicylate"

Lyudmila M Bakaeva, Vladimir V Bakaev

[Worm Breeder's Gazette, 2003]

The purpose of this study was to investigate the effect of sodium salicylate in water solutions on the nematode life span. In this experiment sodium salicylate was used in following dilutions: 1000, 100, 10, 1.0 and 0.1 mg/L. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without sodium salicylate) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without sodium salicylate in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with sodium salicylate in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table

Vladimir V Bakaev et al. (2003) Worm Breeder's Gazette "An attempt to slow aging in C. elegans. 28. A positive effect of acetylsalicylic acid"

Alexey V. Shabalin, Vladimir V Bakaev, Lyudmila M Bakaeva

[Worm Breeder's Gazette, 2003]

The purpose of this study was to investigate the effect of acetylsalicylic acid in water solutions on the nematode life span. In this experiment acetylsalicylic acid was used in following dilutions: 1000, 100, 10, 1.0 and 0.1 mg/L. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without acetylsalicylic acid) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without acetylsalicylic acid in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with acetylsalicylic acid in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table

Vladimir V Bakaev et al. (2003) Worm Breeder's Gazette "An attempt to slow aging in C. elegans. 31. No positive effect of glycerol"

Lyudmila M Bakaeva, Vladimir V Bakaev

[Worm Breeder's Gazette, 2003]

The purpose of this study was to investigate the effect of glycerol in water solutions on the nematode life span. In this experiment glycerol was used in following dilutions: 100 g/L, 10 g/L, 1 g/L, 100 mg/L, 10 mg/L, 1 mg/L and 0.1 mg/L. Three adult animals (3-5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without glycerol) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without glycerol in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with glycerol in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table.

Vladimir V Bakaev et al. (2003) Worm Breeder's Gazette "An attempt to slow aging in C. elegans. 30. No positive effect of kudesan"

Lyudmila M Bakaeva, Vladimir V Bakaev

[Worm Breeder's Gazette, 2003]

The purpose of this study was to investigate the effect of kudesan (a water-soluble medicine, containing 30.0 mg of ubiquinone and 4.5 mg of a-tocopherol in 1 mL) in water solutions on the nematode life span. In this experiment kudesan was used in following dilutions: 1000, 100, 10, 1.0 and 0.1 mg/L. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without kudesan) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without kudesan in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with kudesan in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table.

Vladimir V Bakaev et al. (2002) Worm Breeder's Gazette "An attempt to slow aging in C. elegans. 27. A positive effect of acetylsalicylic acid"

Alexey V Shabalin, Lyudmila M Bakaeva, Vladimir V Bakaev

[Worm Breeder's Gazette, 2002]

The purpose of this study was to investigate the effect of acetylsalicylic acid in water solutions on the nematode life span. In this experiment acetylsalicylic acid was used in following dilutions: 1000, 100, 10, 1.0 and 0.1 mg/L. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without acetylsalicylic acid) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without acetylsalicylic acid in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with acetylsalicylic acid in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Conclusion: If acetylsalicylic acid solution was applied to C. elegans in concentration of 10 mg/L, it was able to increase significantly (P>0.05) their mean longevity in comparison with control to 83.96 percent.

Vladimir V Bakaev et al. (2003) Worm Breeder's Gazette "An attempt to slow aging in C. elegans. 32. No positive effect of peroxide"

Vladimir V Bakaev, Lyudmila M Bakaeva

[Worm Breeder's Gazette, 2003]

The purpose of this study was to investigate the effect of hydrogen peroxide in water solutions on the nematode life span. In this experiment hydrogen peroxide was used in following dilutions: 100 mg/L, 10 mg/L, 1 mg/L, 0.1 mg/L, 0.01 mg/L and 0.001 mg/L. Three adult animals (3-5 days old) were kept in microtitre wells containing 0.5 ml of liquid medium (with E. coli and without hydrogen peroxide) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without hydrogen peroxide in medium) every day (one worm in one well) beginning from third day. Then, beginning from 3rd day, these worms were transferred every day in next wells containing medium with hydrogen peroxide in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Conclusion: If hydrogen peroxide solution was applied to C. elegans, it was not able to increase their mean longevity significantly in comparison with control.

Bakaev VV (1996) Worm Breeder's Gazette "AN ATTEMPT TO SLOW AGING IN C. elegans. 3.THE EFFECT OF EXTRACT FROM YOUNG WORMS DURING POSTREPRODUCTIVE PERIOD"

Bakaev VV

[Worm Breeder's Gazette, 1996]

C. elegans (1 - 2 days old) were collected from separate stock, frozen and after 12 hours or more time, thawed, filtrated and mixed with S medium containing E. coli (1:1), this mixture was used as a medium for worms in experimental group. The medium for control group was prepared by mixing S medium containing E. coli with S medium without E. coli (1:1). Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0,75 ml of liquid medium (with E. coli and with or without young worms extract) during 12 hours, then they were discarded and newborn larvae were transferred in next wells every day (one worm in one well). The medium with extract from young worms was used in experimental group beginning from 12th day, that is practically in postreproductive period of nematode life span. A number of progeny was calculated every day. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table.

Bakaev VV (2000) Worm Breeder's Gazette "An attempt to slow aging in C. elegans. 16. no effect of kanamycin"

Bakaev VV

[Worm Breeder's Gazette, 2000]

The purpose of this study was to investigate the effect of different concentrations of kanamycin sulphate in water solutions on nematode life span. In this experiment kanamycin sulphate was used in following dilutions: 1:101, 1:102, 1:103, 1:104, 1:105, 1:106 and 1:107. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0,75 ml of liquid medium (with E. coli and without kanamycin sulphate) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with kanamycin sulphate in any concentration) every day (one worm in one well) beginning from third day. This investigation was carried out in temperature +210C and in the darkness.

Bakaev VV (2000) Worm Breeder's Gazette "An attempt to slow aging in C. elegans. 15. no effect of rifampicin"

Bakaev VV

[Worm Breeder's Gazette, 2000]

The purpose of this study was to investigate the effect of different concentrations of rifampicin in water solutions on nematode life span. In this experiment rifampicin was used in following dilutions: 1:104, 1:105, 1:106, 1:107, 1:108, 1:109 and 1:1010. Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0,75 ml of liquid medium (with E. coli and without rifampicin) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with rifampicin in any concentration) every day (one worm in one well) beginning from third day. This investigation was carried out in temperature +210C and in the darkness.