Zarkower D et al. (1994) Worm Breeder's Gazette "mab-3 YAC Rescue"

De Bono M, Hodgkin JA, Zarkower D

[Worm Breeder's Gazette, 1994]

mab-3 YAC rescue David Zarkower, Mario de Bono, and Jonathan Hodgkin MRC Laboratory of Molecular Biology, Cambridge, England

Weinkove D (2018) BMC Biol "On microbes, aging and the worm: an interview with David Weinkove."

Weinkove D

[BMC Biol, 2018]

David Weinkove is an associate professor at Durham University, UK, studying host-microbe interactions in the model organism Caenorhabditis elegans. David has been focusing on the way microbes affect the physiology of their hosts, including the process of aging. In this interview, he discusses the questions shaping his research, how they evolved over the years, and his guiding principles for leading a lab.

Miller DM et al. (1992) Worm Breeder's Gazette "unc-4 LacZ Expression in A-Type Motor Neurons"

Miller DM, Niemeyer CJ

[Worm Breeder's Gazette, 1992]

unc-4 LacZ expression in A-type motor neurons David M. Miller and Charles J. Niemeyer, Dept. of Cell Biology, Duke Univ. Medical Ctr, Durham, NC 27710

Sanders, Matthew J. et al. (2009) International Worm Meeting "Interactions between insulin/IGF signaling and AMP-activated protein kinase in C. elegans ageing."

Sanders, Matthew J., McElwee, Josh, Gems, David, Carling, David, Schuster, Eugene

[International Worm Meeting, 2009]

Reduced insulin/IGF signaling (IIS) increases lifespan and this effect is dependent upon the DAF-16 FoxO transcription factor. However, the processes regulated by IIS/FoxO that directly control aging remain poorly understood. Previous studies have shown that genes involved in metabolism, stress resistance, cell cycle arrest and apoptosis are directly regulated by FoxO. The longevity of daf-2 insulin/IGF receptor mutants is also dependent upon AMP-activated protein kinase (AMPK)1,2. This enzyme acts as a fuel gauge of the cell3: When the AMP:ATP ratio increases, AMPK is activated and phosphorylates key metabolic enzymes, resulting in increased catabolism and reduced biosynthesis. AMPK is a heterotrimer with a catalytic a-subunit, and regulatory b- and g-subunits3. Binding of AMP to the g-subunit activates AMPK. There is evidence that DAF-16 acts downstream of AMPK, e.g. the longevity resulting from an AMPK activation mutation is suppressed by mutation of daf-16, and AMPK phosphorylates DAF-16 (ref. 4). In an attempt to identify direct targets of DAF-16, DamID (DNA adenine methyltransferase identification) was used in combination with microarray analysis (see abstract by E. Schuster et al.). Results using this approach imply that two of the five predicted AMPK g subunits are up-regulated by DAF-16. Moreover, microarray data5,6 reveals up-regulation of an a-subunit gene, aak-2 and a b-subunit gene, aakb-1, in daf-2 relative to daf-16; daf-2. Thus, DAF-16 is predicted to increase expression of the entire AMPK heterotrimer. This suggests action of AMPK downstream as well as upstream of DAF-16, and the presence of a positive feedback loop. We are now investigating further the relationship between IIS and AMPK in the control of aging in C. elegans. 1. Apfeld et al. Genes Dev. 18, 3004-9 (2004). 2. Curtis et al. Aging Cell 5, 119-26 (2006). 3. Hardie & Carling. Eur. J. Biochem. 246, 259-273 (1997). 4. Greer et al. Curr. Biol. 17, 1646-56 (2007). 5. McElwee et al. Genome Biol. 8, R132 (2007). 6. McElwee et al. J. Biol. Chem. 279, 44533-43 (2004)..

Grenache DG et al. (1993) Worm Breeder's Gazette "Differential Effects of Dauer-Defective Mutations in L1-Specific Surface Antigen Switching"

Politz SM, Grenache DG

[Worm Breeder's Gazette, 1993]

DIFFERENTIAL EFFECTS OF DAUER-DEFECTIVE MUTATIONS ON L1- SPECIFIC SURFACE ANTIGEN SWITCHING. David G. Grenache and Samuel M. Politz, Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA.

Emmons SW et al. (1994) Worm Breeder's Gazette "Strain Names for non-C. elegans Species."

Emmons SW, Fitch DHA, Leroi AM

[Worm Breeder's Gazette, 1994]

Strain names for non-C. elegans species Scott W. Emmonst, Armand Leroit, and David Fitch, Department of Molecular Genetics, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461, Department of Biology, New York University, RmlOO9 Main Bldg., Washington Square, New York, NY 10003

Hall DH et al. (1994) Worm Breeder's Gazette "Cytology of Degenerin-Induced Cell Death in the PVM Neuron"

Driscoll MA, Chalfie M, Gu GQ, Hall DH, Gong L

[Worm Breeder's Gazette, 1994]

Cytology of degenerin-induced cell death in the PVM neuron David H. Hall, Guoqiang Gu+, Lei Gong#, Monica Driscoll#, and Martin Chalfie+, * Dept. Neuroscience, Albert Einstein College of Medicine, Bronx, N.Y. 10461 + Dept. Biological Sciences, Columbia University, New York, N.Y. 10027 # Dept. Molecular Biology and Biochemistry, Rutgers University, Piscataway, N.J. 08855

Amrit FRG et al. (2017) J Vis Exp "Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project."

Ghazi A, Amrit FRG

[J Vis Exp, 2017]

Next generation sequencing (NGS) technologies have revolutionized the nature of biological investigation. Of these, RNA Sequencing (RNA-Seq) has emerged as a powerful tool for gene-expression analysis and transcriptome mapping. However, handling RNA-Seq datasets requires sophisticated computational expertise and poses inherent challenges for biology researchers. This bottleneck has been mitigated by the open access Galaxy project that allows users without bioinformatics skills to analyze RNA-Seq data, and the Database for Annotation, Visualization, and Integrated Discovery (DAVID), a Gene Ontology (GO) term analysis suite that helps derive biological meaning from large data sets. However, for first-time users and bioinformatics' amateurs, self-learning and familiarization with these platforms can be time-consuming and daunting. We describe a straightforward workflow that will help C. elegans researchers to isolate worm RNA, conduct an RNA-Seq experiment and analyze the data using Galaxy and DAVID platforms. This protocol provides stepwise instructions for using the various Galaxy modules for accessing raw NGS data, quality-control checks, alignment, and differential gene expression analysis, guiding the user with parameters at every step to generate a gene list that can be screened for enrichment of gene classes or biological processes using DAVID. Overall, we anticipate that this article will provide information to C. elegans researchers undertaking RNA-Seq experiments for the first time as well as frequent users running a small number of samples.

Vogel G (2002) Science "Neuroscience. Genes keep neurons' house in order."

Vogel G

[Science, 2002]

As any homeowner knows, timely maintenance is vital for keeping a building functioning properly after construction is finished. The same is evidently true for the complex architecture of the nervous system - at least in the roundworm. On page 686, neuroscientists Oliver Hobert, Oscar Aurelio, and David Hall describe a new family of proteins that help keep the wiring of the worm's nervous system tangle free.

Brownlee DJA et al. (1996) Parasitol Today "Nematode neuropeptides: Localization, isolation and functions."

Holden-Dye L, Brownlee DJA, Walker RJ, Fairweather I

[Parasitol Today, 1996]

Historically, peptidergic substances (in the form of neurosecretions) were linked to moulting in nematodes. More recently, there has been a renewal of interest in nematode neurobiology, initially triggered by studies demonstrating the localization of peptide immunoreactivities to the nervous system. Here, David Brownlee, Ian Fairweather, Lindy Holden-Dye and Robert Walker will review progress on the isolation of nematode neuropeptides and efforts to unravel their physiological actions and inactivation mechanisms. Future avenues for research are suggested and the potential exploitation of peptidergic pathways in future therapeutic strategies