In attempts to find additional alleles of
unc-62 (pka
ceh-25), we tested
e917 on a suggestion from Andrew Chisholm, who had found linkage to LGVL. We confirmed his observations that
e917 is a male-rescuable, maternal-effect, partially penetrant, early larval lethal and showed that it failed to complement the maternal-effect lethal allele
unc-62(
ct344). In experiments to define the
e917 molecular lesion, we identified it as a probable inversion by inverse PCR sequencing of religated Sau3AI fragments of
e917 genomic DNA. Using further direct PCR sequencing we have confirmed the inversion by sequencing across the break points and further defining its structure. One break point is in the
unc-62 region of LGVL, included in cosmid T08H10. The other is on the right arm of V, in a region included in the YAC Y04H4A. The inverted segment near the left break point includes a duplication of 94bp from a region about 700bp to the left of the break point. Therefore the
e917 rearrangement, which was originally isolated following 32P-decay mutagenesis, should be designated C (for complex) rather than In. It is possible that there are additional complex features in the inverted region of LGV, and although the strain we have worked with has been backcrossed 3 times, it could possibly still carry lesions on other chromosomes.