[
BioEssays,
1993]
In trans-splicing, the pre-mRNA products of two different genes are spliced together to form a single, mature mRNA. In one type of trans-splicing, pre-mRNAs of many different genes receive a single, short leader, called spliced leader or SL. This type of trans-splicing was first discovered in the primitive eukaryotes, the trypanosomes, where it is apparently the only kind of nuclear mRNA splicing. Subsequently, it was discovered in nematodes (round worms), trematodes (flat worms), and euglena. Although this type of trans-splicing has never been found in any of the other well-studied organisms, Bruzik and Maniatis have recently reported that mammalian cells are capable of performing the reaction when they are provided with the appropriate pre-mRNAs.