Butler JA et al. (1994) Worm Breeder's Gazette "Looking For Extracellular Matrix Proteinases in C. elegans"

Kramer JM, Butler JA

[Worm Breeder's Gazette, 1994]

LOOKING FOR EXTRACELLULAR MATRIX PROTEINASES IN C. ELEGANS. James A. Butler and James M. Kramer, Northwestern University Medical School. Department of CMS Biology, Chicago IL 60611

Wissmann AK et al. (1994) Worm Breeder's Gazette "Characterization of the let-502 Gene"

Mains PE, Wissmann AK, McGhee JD

[Worm Breeder's Gazette, 1994]

Characterization of the let-502 gene Andreas Wissmann, James D. McGhee and Paul E. Mains, Dept. of Medical Biochemistry, University of Calgary, Calgary, Alberta, Canada T2N 4N1

Maske B et al. (1994) Worm Breeder's Gazette "Localization of SKN-1 Protein to Posterior Blastomere in the Early C. elegans Embryo"

Maske B, Bowerman BA, Lommel S

[Worm Breeder's Gazette, 1994]

LOCALIZATION OF SKN-1 PROTEIN TO POSTERIOR BLASTOMERES IN THE EARLY C. ELEGANS EMBRYO. Bill Maske, Silvi Lommel, and Bruce Bowerman. Institute of Molecular Biology, University of Oregon, 97403.

Shoman LM et al. (1994) Worm Breeder's Gazette "Worm Community System Users Envision Future Application."

Grossman E, Shoman LM, Jamison DC, Schatz BR

[Worm Breeder's Gazette, 1994]

Worm Community System Users Envision Future Application. Laura Shoman, Curt Jamison, Ed Grossman, Bruce Schatz, Community Systems Laboratory, National Center for Supercomputing Applications, University of Illinois, Urbana- Champaign, 405 North Mathews, Urbana, IL 61801 (wcs@csl.ncsa.uiuc.edu)

Chamberlain SH et al. (1994) Worm Breeder's Gazette "A Molecular Approach to Identify skn-1-Responsive Genes in the Early C. elegans Embryo"

Chamberlain SH, Bowerman BA

[Worm Breeder's Gazette, 1994]

A molecular approach to identify skn-1-responsive genes in the early C. elegans embryo. Stephen H. Chamberlain and Bruce Bowerman, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403

Bowerman BA et al. (1994) Worm Breeder's Gazette "The Maternal Gene skn-4 and the Specification of Ventral Blastomere Fates in the Early C. elegans Embryo"

Bowerman BA, Shelton CA, Thorpe CJ, Martin PR

[Worm Breeder's Gazette, 1994]

THE MATERNAL GENE SKN-4 AND THE SPECIFICATION OF VENTRAL BLASTOMERE FATES IN THE EARLY C. ELEGANS EMBRYO Bruce Bowerman, Paula R. Martin, Christopher J. Thorpe, and Christopher A. Shelton. The Institute of Molecular Biology, University of Oregon, Eugene, OR 97403.

Sabrina G C Shore et al. (2002) West Coast Worm Meeting "The temperature sensitive lethal mutation or566 may define a new gene that functions in early morphogenesis"

Andrew D Chisholm, Sarah L Moseley, Sabrina G C Shore

[West Coast Worm Meeting, 2002]

Mutations in the EPH receptor tyrosine kinase VAB1 and in its ephrin ligands cause incompletely penetrant defects in gastrulation cleft closure and epidermal enclosure. Fully penetrant defects in cleft closure are seen when multiple pathways are blocked (e.g. vab-1 and ptp-3 (Harrington et al., 2002, Development 129: 2141). To find genes that may have essential functions in gastrulation cleft closure we have begun to examine conditional lethal mutants for possible defects in this process. Bruce Bowermans lab identified several new temperature-sensitive (ts)-lethal mutations affecting morphogenesis in their large scale ts-lethal screens, and very kindly provided some of them to us.

Itani, Omar et al. (2019) International Worm Meeting "Translational machinery determines hypoxic survival in C. elegans."

Bruce, James, van Gilst, Marc, Scott, Barbara, Crowder, Mike, Yan, Jun Yi, Flibotte, Stephane, Itani, Omar, Zhong, Xuefei

[International Worm Meeting, 2019]

We are interested in identifying genes that regulate cellular response to and survival from hypoxia. To that end, we performed a forward ENU genetic screen to identify genes necessary for hypoxic death. We identified six conserved genes; five of these genes (pept-1, tars-1, rtcb-1, xpo-3 and ddx-52) are predicted to be necessary for normal global protein synthesis. To understand how alterations in protein synthesis lead to hypoxia resistance, we performed a suppressor screen using a temperature sensitive growth arrest phenotype of ddx-52(lf). DDX-52 is an RNA helicase involved in ribosome biogenesis. Identified suppressors include genes involved in: repression of translation, desumoylation of proteins and regulators of ribosome biogenesis. In addition to suppressing hypoxia resistance of ddx-52(lf), mutations in repressors of translation larp-1 (encodes an RNA binding protein) and ncl-1 (encodes a B-box zinc finger protein) suppressed the hypoxia resistance of mutants involved in tRNA metabolism (tars-1, rtcb-1 and xpo-3). To determine if ncl-1(lf) and larp-1(lf) are regulating translation rate of tars-1(rf) (encoding threonyl tRNA synthetase) animals, we measured translation rate using SILAC incorporation. While tars-1(rf) animals had reduced global translation rate compared to wild type and to larp-1(lf) ncl-1(lf) animals, the global translation rate of tars-1(rf); larp-1(lf) ncl-1(lf) animals was not different from tars-1(rf) animals. Thus, translation rate per se does not determine hypoxic sensitivity. We propose that multicellular organisms have evolved mechanisms of sensing and coordinating translation capacity for various physiological functions. These mechanisms may insure the survival of animals exposed to stressors that cause reduced translation. Our data demonstrate the crucial role that translation mechanisms have in hypoxic sensitivity. The translation machinery involves hundreds of genes, but our genetic approach allows us to identify specific factors that serve as regulatory hot spots in the overall control of translation and hypoxic survival.

Schnabel H et al. (1986) Worm Breeder's Gazette "threee ts alleles of e2072."

Schnabel R, Schnabel H

[Worm Breeder's Gazette, 1986]

Three of the ts embryonic lethal mutants (e2141, e2142, and e2144) lack the anterior half of the pharynx but make the posterior half. They are allelic to each other and to e2072, a maternal effect embryonic lethal mutant isolated by James Priess (C. elegans meeting 1985, p.16). All of them are defective in the anterior part of the pharynx, which is normally derived from AB, whereas the posterior part, normally derived from MS, forms a rather normal posterior pharynx bulb. All 3 alleles have a temperature-sensitive period during early embryogenesis, suggesting that a maternally supplied product is exerting its function at this time. Two of the three (e2141 and e2144) have a second, postembryonic ts phase causing sterility, i.e. adults do not produce eggs or oocytes. It therefore seems that the wild type function of this gene is required for two not apparently related processes during development.

Sternberg, Paul et al. (2015) International Worm Meeting "WormBase 2015."

Berriman, Matt, Howe, Kevin, Kersey, Paul, Stein, Lincoln, Harris, Todd, Sternberg, Paul, Schedl, Tim

[International Worm Meeting, 2015]

WormBase has existed for 15 years and has evolved in many ways. The new website is fully operational and has made the process of adding new data types, displays, and tools easier. Behind the scenes we are piloting an overhaul of the underlying database infrastructure to allow us to handle the ever increasing data, have the website perform faster, and allow more frequent updates of information. This is a critical time for the project, as we face considerable pressure from two directions. The first is that our funders really want us to do more with less. We are responding to this by leading the way in making curation (the process of extracting information from papers and data sets into computable form) more efficient using a new version of Textpresso (to be released later this calendar year); by discussing with other model organism information resources ways to work together to be more efficient and inter-connected; and by seeking additional sources of funding. The second, delightful, pressure is an increase in data and results generated by the C. elegans and nematode communities. While we are handling this increase by changes in our software for curation, the database infrastructure, and the website, we do need your help. Many of you have helped us over the last few years to identify data in your papers or by sending us data directly. We now need you to help with a few types of information by submitting the data via specially designed, user-friendly forms that ensure good quality and the use of standard terminology. In particular, we have a large backlog of uncurated information associating alleles with phenotypes. We pledge to make this process as painless as possible, and to improve WormBase's description of phenotypes with your feedback, starting at this meeting at the WormBase booth, workshops and posters. With your help, continual improvement of our efficiency, and additional sources of funding, we are optimistic that we can do much more with even somewhat less effort.Consortium: Paul Davis, Michael Paulini, Gary Williams, Bruce Bolt, Thomas Down, Jane Lomax, Todd Harris, Sibyl Gao, Scott Cain, Xiaodong Wang, Karen Yook, Juancarlos Chan, Wen Chen, Chris Grove, Mary Ann Tuli, Kimberly Van Auken, D. Wang, Ranjana Kishore, Raymond Lee, John DeModena, James Done, Yuling Li, H.-M. Mueller, Cecilia Nakamura, Daniela Raciti, Gary Schindelman.