Correlation of the Genetic and Phvsical maps within the
rol-3 region of LGV (left) W. Bradley Barbazuk and David L. Baillie Institute of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC. CANADA, VSA 1 S6 In an attempt to identify the physical location of the
rol-3 (V) gene we have begun the systematic germ-line transformation rescue of lethal mutations within zones 16-18 of LGV (JOHNSEN and BAILLIE, GENETICS 129: 735-?52). Using H. Kagawa's placement of
unc-68 (WBG 12(4)) as a starting point we began to inject cosmids to the right of C46C6 into N2 hermaphrodites. In doing so we were able to obtain a battery of strains each carrying cosmids singly, or multiply, in stable arrays which were then used as mini-duplications in subsequent crosses to lethal bearing strains. In total we used eight cosmid bearing strains and one YAC bearing strain in the rescue of 13 lethal mutations including
rol-3 (Table 1). Our germ-line transformation rescue data has enabled us to split up and order some of the lethal mutations within the zone 16-18 gene clusters. (Figure 1). We would like to thank C. Mello and J. Priess for providing a strain carrying an array composed of ZK307, F25G6 and FOlH8, as well as providing purified DNA for many of the cosmids injected.