Miller DM et al. (1992) Worm Breeder's Gazette "unc-4 LacZ Expression in A-Type Motor Neurons"

Miller DM, Niemeyer CJ

[Worm Breeder's Gazette, 1992]

unc-4 LacZ expression in A-type motor neurons David M. Miller and Charles J. Niemeyer, Dept. of Cell Biology, Duke Univ. Medical Ctr, Durham, NC 27710

Sommer RJ et al. (1994) Worm Breeder's Gazette "Evolution of Vulva-Formation: Part II: Species With a Central Vulva"

Sommer RJ, Sternberg PW

[Worm Breeder's Gazette, 1994]

Evolution of vulva-formation: Part II: Species with a central vulva Ralf J. Sommer & Paul W. Sternberg, California Institute of Technology, Division of Biology 156-29, Pasadena, CA 91125

Huang Y-J et al. (1994) Worm Breeder's Gazette "From Ascaris to C. elegans: A Way to Study Gene Structure and Function"

Tobler H, Huang Y-J, Mueller F

[Worm Breeder's Gazette, 1994]

FROM ASCARIS TO C. ELEGANS: A WAY TO STUDY GENE STRUCTURE AND FUNCTION Huang Y-J., Tobler H. and Muller F., Institute of Zoology, University of Pribourg, Perolles, CH-1700 Fribourg, Switzerland

Li WQ et al. (1992) Worm Breeder's Gazette "Characterization of the Axonal Guidance and Outgrowth gene Unc-33"

Shaw JE, Li WQ, Herman RK

[Worm Breeder's Gazette, 1992]

Characterization of the axonal guidance and outgrowth gene unc-33 W. Li, R. K. Herman and J. E. Shaw Department of Genetics and Cell biology, University of Minnesota, St Paul, MN 55108

Almeida CA et al. (1994) Worm Breeder's Gazette "Function of a Domain of the Myosin Heavy Chain Implicated in Familial Hypertrophic Cardiomyopathy"

Collins JJ, Almeida CA, Swift KE

[Worm Breeder's Gazette, 1994]

Function of a Domain of the Myosin Heavy Chain Implicated in Familial Hypertrophic Cardiomyopathy Craig A. Almeida, Kerry E. Swift and John J. Collins Department of Biochemistry and Molecular, University of New Hampshire, Durham, NH 03820

Sommer RJ et al. (1994) Worm Breeder's Gazette "Evolution of Vulva Formation: Part IV: Variation in AC Position Can Cause a Shift of Vulva Formation Towards P(4-6).p."

Sternberg PW, Sommer RJ

[Worm Breeder's Gazette, 1994]

Evolution of vulva formation: Part IV: Variation in AC position can cause a shift of vulva formation towards p(4- 6).p Ralf J. Sommer & Paul W. Sternberg, HHMI & California Institute of Technology, Division of Biology 156-29, Pasadena, CA

Bowerman BA et al. (1994) Worm Breeder's Gazette "The Maternal Gene skn-4 and the Specification of Ventral Blastomere Fates in the Early C. elegans Embryo"

Bowerman BA, Shelton CA, Thorpe CJ, Martin PR

[Worm Breeder's Gazette, 1994]

THE MATERNAL GENE SKN-4 AND THE SPECIFICATION OF VENTRAL BLASTOMERE FATES IN THE EARLY C. ELEGANS EMBRYO Bruce Bowerman, Paula R. Martin, Christopher J. Thorpe, and Christopher A. Shelton. The Institute of Molecular Biology, University of Oregon, Eugene, OR 97403.

Fields CA (1990) Worm Breeder's Gazette "An Updated Codon Usage Table"

Fields CA

[Worm Breeder's Gazette, 1990]

A number of new genes have been sequenced since worm codon usage was summarized by Emmons (Table 2 in The Genome, The Nematode Caenorhabditis ing Harbor Lab.). An updated codon usage table has been derived from the sequences of act-1, act-2, act-3, act-4 (Krause et al., J. Mol. Biol. 208 (1989) 381-392), col-1, col-2 (Kramer et al., Cell 30 (1982) 599-606), col-6, col-7, col-8, col-14, col-19 (Cox et al., Gene 76 (1989) 331-344), deb-1 (Barstead and Waterston, J. Biol. Chem. 264 (1989) 10177-10185), dpy-13 (von Mende et al., Cell 55 (1988) 567-576), glp-1 (Yochem and Greenwald, Cell 58 (1989) 553-563), lin-12 (Yochem et al., Nature 335 (1988) 547- 550), myo-1, myo-2, myo-3 (Dibb et al., J. Mol. Biol. 205 (1989) 603- 613), sqt-1 (Kramer et al., Cell 55 (1988) 555-565), unc-15 (Kagawa et al., J. Mol. Biol. 207 (1989) 311-333), unc-54 (Kam et al., Proc. Natl. Acad. Sci. USA 80 (1983) 42534257), and vit-5 (Spieth et al., Nucl. Acids Res.) [See Figure 1]

Gengyo-Ando K et al. (1989) Worm Breeder's Gazette "sequence analysis of unc-15 mutants and its intragenic revertants."

Kagawa H, Gengyo-Ando K

[Worm Breeder's Gazette, 1989]

To understand the molecular mechanism of thick filament assembly, we are investigating the mutation site of EMS induced unc-15 mutants at the DNA level. These mutant alleles are distinguished two categories. One is e1214, has no detectable paramyosin on SDS-PAGE gels and apparently null phenotype. This mutant had amber nonsense mutation (Q to Amber) near the N-terminus. The others are missense alleles which accumulate normal level's paramyosin, nevertheless result in the disruption of thick filament assembly. The latter alleles are of variable phenotype, the most severely paralyzed allele, e73 caused the reversion of electric charge (E- to K+) by the point mutation. Further sequence analysis of two intragenic e73 revertants revealed that second mutations are exactly in the unc-15 gene, not in a closely linked gene, and also caused the reversion of electric charge (E- to K+). These mutation might not disrupt the secondary structure of paramyosin, but only change the charge on the surface of alpha-helical coiled coil structure. This might mean the assembly of paramyosin itself and/or paramyosin-myosin heavy chain result from charge interactions between the molecules. We hope to calculate what kind of changes are induced by each of substituted amino acid with the computer program exploited by Dr. McLachlan (McLachlan, A. D. and Karn, J,1982, Nature 299, 226-231; McLachlan, A. D. and Karn, J, 1983, J. Mol. Biol. 164, 605-626; Kagawa, H., Gengyo, K., McLachlan, A. D., Brenner, S. and Karn, J., 1989, J. Mol. Biol., 207, 311-333). Molecular organization of unc-15 alleles summarized. More detailed epitope mapping of paramyosin is in progress. [See Figure 1]

Kenyon CJ (1984) Worm Breeder's Gazette "L1 smoothies."

Kenyon CJ

[Worm Breeder's Gazette, 1984]

The widly used CB1490 strain carryinq him-5(e1490) (isolated by J . Hodgkin) has very faint L1 alae. Adult and dauer alae are normal, as are the lateral hypodermal lineages . The Ali- phenotype is due to a linked mutation, ali-1(19B4)V located between dpy-11 and him-5.