Preliminary analysis of six spontaneous
unc-44 alleles indicates that alterations occur over a fairly large region in the genomic DNA. Southern analysis of two alleles from E. Hedgecock (
rh1013 and
rf1042) and four alleles (
mn259,
mn339,
q331, and
st200) from R. Herman and other labs (kindly provided by C. Kari, R. Herman, W. Li, and J. Shaw) indicated that restriction fragment differences from wild type are associated with all six alleles (see Franco et al., C. elegans Meeting abstracts, 1989). The construction of preliminary restriction maps of
unc-44 cosmid (B0350 and C44A, kindly provided by A. Coulson and J. Sulston) and phage (DD#LRF1 and DD#LRF6) clones allows the approximate positioning of mutant changes. The absolute orientations of the cosmid and phage clones are based on positioning of these clones on the cosmid map. The overlapping of the cosmid and phage clones has not yet been demonstrated. Unfortunately, the cosmid isolate that could quickly clarify the situation (C02C3) deleted during growth. [See Figure 1]