[
International Worm Meeting,
2021]
Neurodegenerative diseases such as Alzheimer's disease (AD) are well recognized as major public health problems in the aged population. Recognized events in AD include cognitive impairment, oxidative stress (OS), AB plaques accumulation in brain tissue. The current drugs to treat AD no cure or reduce the progression of the disease. Thus, the study of different extracts can provide a wealth of bioactive compounds and their combination, which can exert a new strategy for several neurodegenerative diseases, including AD. Among the natural extracts, Ginkgo biloba is one of the most investigated herbal remedies for cognitive disorders and AD. Moreover, docosahexaenoic acid (DHA), and Pinitol and Ursolic acid (UA) are associated to a prophylactic role in certain age-related diseases with particular emphasis on some of the effects of certain degenerative diseases. This study aimed to investigate the synergistic neuroprotective effects of mixed extract composed by different concentrations of DHA (150 microM), Ginkgo (120 microM) Pinitol (105 microM), and UA (35 microM) from Biosearch Life product, including OS tolerance, Thioflavin-S staining AB plaques, and lifespan in several transgenic Caenorhabditis elegans (C. elegans) as well as cognitive performance in C. elegans and senescence-accelerated prone mice 8 (SAMP8) model. Firstly, we found a significantly higher survival percentage in C. elegans treated with Mix extract group in comparison with the single extract treated groups (DHA 150 microM group, Ginkgo 120 microM group, Pinitol 105 microM group, UA 35 microM group), reaching the Vitamin C group. Likewise, we found a significantly increased the lifespan in C. elegans Mix extract-treated group compared to the other groups, suggesting the synergistic effects. Remarkably, we determined a significant reduction in AB plaques accumulation in C. elegans strain CL2006 Mix extract group compared to other groups, including all treated groups, confirming the synergistic effect again. Finally, we demonstrated better cognitive performance in the Mix extract group in both AD models (neuronal AB C. elegans strain CL2355 and SAMP8 mice model), confirming the molecular result and demonstrating the synergist effects of this Mix extract. Taken together, our results demonstrated the potential therapeutic strategy for AD of this new Mix extract product from Biosearch Life.