Galindo-Malagn XA et al. (2022) Zootaxa "New species, synonymies and records in the genus Rhagovelia Mayr, 1865 (Hemiptera: Heteroptera: Veliidae) from Colombia."

Moreira FFF, Morales I, Mondragn-F SP, Galindo-Malagn XA

[Zootaxa, 2022]

Rhagovelia medinae sp. nov., of the hambletoni group (angustipes complex), and R. utria sp. nov., of the hirtipes group (robusta complex), are described, illustrated, and compared with similar congeners. Based on the examination of type specimens, six new synonymies are proposed: R. elegans Uhler, 1894 = R. pediformis Padilla-Gil, 2010, syn. nov.; R. cauca Polhemus, 1997 = R. azulita Padilla-Gil, 2009, syn. nov., R. huila Padilla-Gil, 2009, syn. nov., R. oporapa Padilla-Gil, 2009, syn. nov, R. quilichaensis Padilla-Gil, 2011, syn. nov.; and R. gaigei, Drake Hussey, 1947 = R. victoria Padilla-Gil, 2012 syn. nov. The first record from Colombia is presented for R. trailii (White, 1879), and the distributions of the following species are extended in the country: R. cali Polhemus, 1997, R. castanea Gould, 1931, R. cauca Polhemus, 1997, R. gaigei Drake Hussey, 1957, R. elegans Uhler, 1894, R. femoralis Champion, 1898, R. malkini Polhemus, 1997, R. perija Polhemus, 1997, R. sinuata Gould, 1931, R. venezuelana Polhemus, 1997, R. williamsi Gould, 1931, and R. zeteki Drake, 1953.

Wilm TP et al. (1999) Gene "Ballistic transformation of Caenorhabditis elegans."

Schnabel R, Demel P, Koop HU, Wilm TP, Schnabel H

[Gene, 1999]

A novel method to transform the nematode Caenorhabditis elegans is described. DNA coprecipitated with gold particles is shot at worms by means of a helium beam. Transformed worms are either identified by a dominant visible marker or selected by a conditional lethal system.

Ren H et al. (2005) Proc Natl Acad Sci U S A "The crystal structure of human adenylate kinase 6: An adenylate kinase localized to the cell nucleus."

Zhang C, Ren H, Luo M, Liang Y, Bennett M, Lu F, Nan J, Eriksson S, Su XD, Zheng X, Wang L, Li L

[Proc Natl Acad Sci U S A, 2005]

Adenylate kinases (AKs) play important roles in nucleotide metabolism in all organisms and in cellular energetics by means of phosphotransfer networks in eukaryotes. The crystal structure of a human AK named AK6 was determined by in-house sulfur single-wavelength anomalous dispersion phasing methods and refined to 2.0-A resolution with a free R factor of 21.8%. Sequence analyses revealed that human AK6 belongs to a distinct subfamily of AKs present in all eukaryotic organisms sequenced so far. Enzymatic assays show that human AK6 has properties similar with other AKs, particularly with AK5. Fluorescence microscopy showed that human AK6 is localized predominantly to the nucleus of HeLa cells. The identification of a nuclear-localized AK sheds light on nucleotide metabolism in the nucleus and the energetic communication between mitochondria and nucleus by means of phosphotransfer networks.

Benecke M et al. (1996) Electrophoresis "(GTG)5 allows the distinction between different isolates of the nematode Caenorhabditis elegans."

Schierenberg E, Epplen JT, Benecke M

[Electrophoresis, 1996]

We have not been able to distinguish different isolates from the nematode Caenorhabditis elegans by morphological means. However, they differ on the molecular level and strains from several geographic regions can be identified with the help of "genetic fingerprints" using the oligonucleotide probe (GTG)5.

Katz M (2015) Methods Mol Biol "Genetic Methods for Cellular Manipulations in C. elegans."

Katz M

[Methods Mol Biol, 2015]

Neuron manipulation in vivo by ablation, activation, or inactivation, and regulation of gene expression, is essential for dissecting nervous system function. Here we describe genetic means for neuron manipulation in the nematode C. elegans, and provide protocols for generating transgenic animals containing these genetic tools.

Katz M (2022) Methods Mol Biol "Genetic Methods for Cellular Manipulation in C. elegans."

Katz M

[Methods Mol Biol, 2022]

Neuron manipulation in vivo by ablation, activation or inactivation, and regulation of gene expression is essential for dissecting nervous system function. Here we describe genetic means for neuron manipulation in the nematode C. elegans, and provide protocols for generating transgenic animals containing these genetic tools.

Katju V et al. (2008) Genetics "Sex change by gene conversion in a Caenorhabditis elegans fog-2 mutant."

Katju V, Lipinski KJ, Bergthorsson U, Labeau EM

[Genetics, 2008]

Caenorhabditis elegans primarily reproduces as a hermaphrodite. Independent gene conversion events in mutant obligately outcrossing populations of C. elegans (fog-2 (lf)) spontaneously repaired the loss of function mutation in the fog-2 locus, thereby reestablishing hermaphroditism as the primary means of reproduction for the populations.

Cutter AD (2008) Curr Biol "Reproductive evolution: symptom of a selfing syndrome."

Cutter AD

[Curr Biol, 2008]

In the nematode Caenorhabditis elegans, a single gene (plg-1) encodes the dominant protein found in mating plugs - a means of inhibiting multiple matings. Naturally occurring loss of plg-1 function results in males that fail to deposit mating plugs - a manifestation of relaxed sexual selection since the evolution of self-fertilization in this species.

Inoue M et al. (2015) Nat Methods "Rational design of a high-affinity, fast, red calcium indicator R-CaMP2."

Takemoto-Kimura S, Kitamura K, Nakai J, Kamijo S, Fujii H, Kano M, Gengyo-Ando K, Ohkura M, Horigane SI, Bito H, Takeuchi A, Inoue M

[Nat Methods, 2015]

Fluorescent Ca(2+) reporters are widely used as readouts of neuronal activities. Here we designed R-CaMP2, a high-affinity red genetically encoded calcium indicator (GECI) with a Hill coefficient near 1. Use of the calmodulin-binding sequence of CaMKK- and CaMKK- in lieu of an M13 sequence resulted in threefold faster rise and decay times of Ca(2+) transients than R-CaMP1.07. These features allowed resolving single action potentials (APs) and recording fast AP trains up to 20-40 Hz in cortical slices. Somatic and synaptic activities of a cortical neuronal ensemble in vivo were imaged with similar efficacy as with previously reported sensitive green GECIs. Combining green and red GECIs, we successfully achieved dual-color monitoring of neuronal activities of distinct cell types, both in the mouse cortex and in freely moving Caenorhabditis elegans. Dual imaging using R-CaMP2 and green GECIs provides a powerful means to interrogate orthogonal and hierarchical neuronal ensembles in vivo.

Harnpicharnchai P et al. (2001) Mol Cell "Composition and functional characterization of yeast 66S ribosome assembly intermediates."

Horsey E, Brame CJ, Rout M, Jakovljevic J, Imai B, Woolford JL, Roman J, Shabanowitz J, Hunt DF, Guo Y, Harnpicharnchai P, Meagher D, Miles T

[Mol Cell, 2001]

The pathway and complete collection of factors that orchestrate ribosome assembly are not clear. To address these problems, we affinity purified yeast preribosomal particles containing the nucleolar protein Nop7p and developed means to separate their components. Nop7p is associated primarily with 66S preribosomes containing either 27SB or 25.5S plus 7S pre-rRNAs. Copurifying proteins identified by mass spectrometry include ribosomal proteins, nonribosomal proteins previously implicated in 60S ribosome biogenesis, and proteins not known to be involved in ribosome production. Analysis of strains mutant for eight of these proteins not previously implicated in ribosome biogenesis showed that they do participate in this pathway. These results demonstrate that proteomic approaches in concert with genetic tools provide powerful means to purify and characterize ribosome assembly intermediates.