dpy-22 : pop-1

In dpy-22 (n=6) animals, the levels of POP-1 were higher in the anterior T-cell daughter, just as in wild-type animals.

let-19 : pop-1

In let-19 (n=6) animals, the levels of POP-1 were higher in the anterior T-cell daughter, just as in wild-type animals.

ced-3 : lin-28

"CED-3 cleavage of LIN-28 generates an Arg/N-End rule N-degron."

apr-1 : pry-1

In pry-1 mutants, APR-1::GFP was symmetrically localized to the cortex of the seam cells, except for the T cell, prior to their division (80%; n = 20 for V5.p), suggesting that pry-1 also regulates the cortical localization of APR-1. On the other hand, in the T cell, where cortical PRY-1 was not asymmetric, APR-1::GFP localization was not affected in pry-1 mutants.

mig-10 : unc-34

"The N-terminal 118 amino acids of MIG-10A (MIG-10A-N) binds the 60 KD UNC-34."

apr-1 : pry-1

In pry-1 mutants, APR-1::GFP was symmetrically localized to the cortex of the seam cells, except for the T cell, prior to their division (80%; n = 20 for V5.p), suggesting that pry-1 also regulates the cortical localization of APR-1. On the other hand, in the T cell, where cortical PRY-1 was not asymmetric, APR-1::GFP localization was not affected in pry-1 mutants.

clp-1 : mec-4

Dr. N. Tavernarakis: suppression of neurodegeneration

Interaction involving hlh-14

"To test whether hlh-14 mutants have extra T cells, we used a C50B6.8::gfp reporter. C50B6.8 is a C. elegans gene that is expressed in the ten hypodermal seam cells, including the T cells (Mounsey et al., 2002) (Fig. 5C)... With this GFP reporter, we observed that hlh-14 mutants often have extra GFP-expressing cells in the tail, presumably extra T (or T-like) cells (Fig. 5D)... Notably, these supernumerary T-like cells are smaller than normal T cells (Fig. 5D)."

lin-44 : tlp-1

"We examined the expression of tlp-1::gfp in a lin-44 mutant to determine whether TLP-1 localization might respond to LIN-44 polarity signals. We observed weak GFP expression in the T cell in 8% (n=108) of lin-44 animals (not shown). Interestingly, we found that tlp-1::gfp expression is often reversed in lin-44 mutants, reflecting the reversal in T cell polarity (Fig 6A). Specifically, of the animals in which we observed GFP expression in the T cell lineage (20/86), 60% showed expression in T.a only, 20% showed expression in T.p only, and 20% showed expression in T.a and T.p. These results correlate with the effect of lin-44 mutations on the T cell division: roughly 70% of the T cell divisions are reversed, 20% are symmetric and 10% are normal (Herman and Horvitz, 1994)."

cdc-37 : hsp-90

"CeCdc37 binds Hsp90 via its N-domain."