6 Interactions found (0.008 seconds)

Proteins associated with either an AIN-1::GFP or an AIN-2::GFP protein, each expressed from an integrated functional transgene, were precipitated with an anti-GFP antibody (Ding et al., 2005) and analyzed on a mass spectrometer using the Multidimensional Protein Identification Technology (Mud-PIT) (Washburn et al., 2001). ALG-1 and ALG-2, the two Argonaute proteins that share functions in the miRNA pathway (Grishok et al., 2001), were the most abundant proteins detected in either AIN-1-or AIN-2-associated complexes but were absent in the control samples (Figure 2F). Conversely, we also performed CoIP using GFP-tagged ALG-1 and ALG-2 proteins (Figure 2G). AIN-1 was found to coprecipitate with both in a western blot analysis using a newly raised anti-AIN-1 antibody. The miRISC-specific ALG-1 and ALG-2 were the only two Argonaute proteins detected in either AIN-1 or AIN-2 CoIP samples, indicating that AIN-1 and AIN-2 are likely to be specifically involved in the miRNA pathway. Meanwhile, AIN-1 was not detected in the AIN-2 IP sample, and vice versa, indicating that AIN-1 and AIN-2 are in distinct complexes and supporting the idea that AIN-1 and AIN-2 are redundant components of miRISCs.

Proteins associated with either an AIN-1::GFP or an AIN-2::GFP protein, each expressed from an integrated functional transgene, were precipitated with an anti-GFP antibody (Ding et al., 2005) and analyzed on a mass spectrometer using the Multidimensional Protein Identification Technology (Mud-PIT) (Washburn et al., 2001). ALG-1 and ALG-2, the two Argonaute proteins that share functions in the miRNA pathway (Grishok et al., 2001), were the most abundant proteins detected in either AIN-1-or AIN-2-associated complexes but were absent in the control samples (Figure 2F). Conversely, we also performed CoIP using GFP-tagged ALG-1 and ALG-2 proteins (Figure 2G). AIN-1 was found to coprecipitate with both in a western blot analysis using a newly raised anti-AIN-1 antibody. The miRISC-specific ALG-1 and ALG-2 were the only two Argonaute proteins detected in either AIN-1 or AIN-2 CoIP samples, indicating that AIN-1 and AIN-2 are likely to be specifically involved in the miRNA pathway. Meanwhile, AIN-1 was not detected in the AIN-2 IP sample, and vice versa, indicating that AIN-1 and AIN-2 are in distinct complexes and supporting the idea that AIN-1 and AIN-2 are redundant components of miRISCs.

Proteins associated with either an AIN-1::GFP or an AIN-2::GFP protein, each expressed from an integrated functional transgene, were precipitated with an anti-GFP antibody (Ding et al., 2005) and analyzed on a mass spectrometer using the Multidimensional Protein Identification Technology (Mud-PIT) (Washburn et al., 2001). ALG-1 and ALG-2, the two Argonaute proteins that share functions in the miRNA pathway (Grishok et al., 2001), were the most abundant proteins detected in either AIN-1-or AIN-2-associated complexes but were absent in the control samples (Figure 2F). Conversely, we also performed CoIP using GFP-tagged ALG-1 and ALG-2 proteins (Figure 2G). AIN-1 was found to coprecipitate with both in a western blot analysis using a newly raised anti-AIN-1 antibody. The miRISC-specific ALG-1 and ALG-2 were the only two Argonaute proteins detected in either AIN-1 or AIN-2 CoIP samples, indicating that AIN-1 and AIN-2 are likely to be specifically involved in the miRNA pathway. Meanwhile, AIN-1 was not detected in the AIN-2 IP sample, and vice versa, indicating that AIN-1 and AIN-2 are in distinct complexes and supporting the idea that AIN-1 and AIN-2 are redundant components of miRISCs.

Proteins associated with either an AIN-1::GFP or an AIN-2::GFP protein, each expressed from an integrated functional transgene, were precipitated with an anti-GFP antibody (Ding et al., 2005) and analyzed on a mass spectrometer using the Multidimensional Protein Identification Technology (Mud-PIT) (Washburn et al., 2001). ALG-1 and ALG-2, the two Argonaute proteins that share functions in the miRNA pathway (Grishok et al., 2001), were the most abundant proteins detected in either AIN-1-or AIN-2-associated complexes but were absent in the control samples (Figure 2F). Conversely, we also performed CoIP using GFP-tagged ALG-1 and ALG-2 proteins (Figure 2G). AIN-1 was found to coprecipitate with both in a western blot analysis using a newly raised anti-AIN-1 antibody. The miRISC-specific ALG-1 and ALG-2 were the only two Argonaute proteins detected in either AIN-1 or AIN-2 CoIP samples, indicating that AIN-1 and AIN-2 are likely to be specifically involved in the miRNA pathway. Meanwhile, AIN-1 was not detected in the AIN-2 IP sample, and vice versa, indicating that AIN-1 and AIN-2 are in distinct complexes and supporting the idea that AIN-1 and AIN-2 are redundant components of miRISCs.

Proteins associated with either an AIN-1::GFP or an AIN-2::GFP protein, each expressed from an integrated functional transgene, were precipitated with an anti-GFP antibody (Ding et al., 2005) and analyzed on a mass spectrometer using the Multidimensional Protein Identification Technology (Mud-PIT) (Washburn et al., 2001). ALG-1 and ALG-2, the two Argonaute proteins that share functions in the miRNA pathway (Grishok et al., 2001), were the most abundant proteins detected in either AIN-1-or AIN-2-associated complexes but were absent in the control samples (Figure 2F). Conversely, we also performed CoIP using GFP-tagged ALG-1 and ALG-2 proteins (Figure 2G). AIN-1 was found to coprecipitate with both in a western blot analysis using a newly raised anti-AIN-1 antibody. The miRISC-specific ALG-1 and ALG-2 were the only two Argonaute proteins detected in either AIN-1 or AIN-2 CoIP samples, indicating that AIN-1 and AIN-2 are likely to be specifically involved in the miRNA pathway. Meanwhile, AIN-1 was not detected in the AIN-2 IP sample, and vice versa, indicating that AIN-1 and AIN-2 are in distinct complexes and supporting the idea that AIN-1 and AIN-2 are redundant components of miRISCs.

Proteins associated with either an AIN-1::GFP or an AIN-2::GFP protein, each expressed from an integrated functional transgene, were precipitated with an anti-GFP antibody (Ding et al., 2005) and analyzed on a mass spectrometer using the Multidimensional Protein Identification Technology (Mud-PIT) (Washburn et al., 2001). ALG-1 and ALG-2, the two Argonaute proteins that share functions in the miRNA pathway (Grishok et al., 2001), were the most abundant proteins detected in either AIN-1-or AIN-2-associated complexes but were absent in the control samples (Figure 2F). Conversely, we also performed CoIP using GFP-tagged ALG-1 and ALG-2 proteins (Figure 2G). AIN-1 was found to coprecipitate with both in a western blot analysis using a newly raised anti-AIN-1 antibody. The miRISC-specific ALG-1 and ALG-2 were the only two Argonaute proteins detected in either AIN-1 or AIN-2 CoIP samples, indicating that AIN-1 and AIN-2 are likely to be specifically involved in the miRNA pathway. Meanwhile, AIN-1 was not detected in the AIN-2 IP sample, and vice versa, indicating that AIN-1 and AIN-2 are in distinct complexes and supporting the idea that AIN-1 and AIN-2 are redundant components of miRISCs.