lbp-8 : nape-1

The nape-1(tm3860) loss-of-function mutation suppressed the life-span extension in lipl-4 Tg and lbp-8 Tg by half (fig. S13 and table S1).

lipl-4 : nape-1

The nape-1(tm3860) loss-of-function mutation suppressed the life-span extension in lipl-4 Tg and lbp-8 Tg by half (fig. S13 and table S1).

acs-2 : lbp-8

acs-2 mRNA amount is increased in lbp-8 Tg but not in thetransgenic strain expressing lbp-8 without the N-terminal NLS(lbp-8 Tg no NLS).

lbp-8 : lipl-4

lbp-8 mRNA amounts were increased in lipl4 Tg compared with WT but not in the transgenic strain overexpressing lipl-4 that lacks the signal peptide for lysosomal expression (lipl-4 Tg no SP).

orai-1 : stim-1

"Upon TG (thapsigargin) treatment, we observed clear PM-localized puncta of GFP-C.STIM1 and Cherry-C.Orai1."

lbp-8 : lipl-4

a loss-of-function mutant lipl-4(tm4417) reduced the longevity of lbp-8 Tg by 68%(fig. S14),

eat-2 : lipl-4

Concordantly, the longevity extensions by lipl-4 Tg and eat-2(ad1116), a genetic model of dietary restriction in C. elegans (13), were additive (fig. S10).

lbp-8 : lipl-4

lbp-8(rax1), suppressed the life-span extension in lipl-4 Tg animals without affecting WT life span (Fig. 2H, fig. S7, and table S1).

lbp-8 : nhr-49

nhr-49 RNAi reduced the life span of long-lived lbp-8-overexpressing transgenic (Tg) worms to that of short-lived nhr-49(RNAi) worms, suggesting that nhr-49(RNAi) is epistatic to lbp-8 overexpression

lipl-4 : nhr-49

nhr-49 RNAi reduced the life span of long-lived lipl-4-overexpressing transgenic (Tg) worms to that of short-lived nhr-49(RNAi) worms, suggesting that nhr-49(RNAi) is epistatic to lipl-4 overexpression