"Pharmacological characterization revealed that the receptor is potently activated by flp-2 peptides in CHO cells (~10 nM EC50) and in yeast (~100 nM EC50), signaling through a Gqalpha pathway in each system."
"Application of either NLP-12-1 or NLP-12-2 synthetic peptides produced robust calcium responses in cells expressing CKR-1. These responses were concentration-dependent with EC50 values of 3.5 and 1.9 nM for NLP-12-1 and NLP-12-2 peptides, respectively. These EC50 values are comparable to those measured for NLP-12 activation of CKR-2 (8.0 nM and 10.2 nM), suggesting NLP-12 peptides act with similar potency across CKR-1 and CKR-2 GPCRs."
"Application of either NLP-12-1 or NLP-12-2 synthetic peptides produced robust calcium responses in cells expressing CKR-1. These responses were concentration-dependent with EC50 values of 3.5 and 1.9 nM for NLP-12-1 and NLP-12-2 peptides, respectively. These EC50 values are comparable to those measured for NLP-12 activation of CKR-2 (8.0 nM and 10.2 nM), suggesting NLP-12 peptides act with similar potency across CKR-1 and CKR-2 GPCRs."
"After cloning and expressing Ce-PK-R in HEK293T cells,we found that it was activated by a neuropeptide from the C. elegans NLP-44 precursor (EC50 = 18 nM)."
SYD-2::GFP fusion protein is localized to discrete puncta of 200-400 nm in diameter along the dorsal and ventral nerve cord. The size of SYD-2::GFP puncta was increased in goa-1 mutants.
SYD-2::GFP fusion protein is localized to discrete puncta of 200-400 nm in diameter along the dorsal and ventral nerve cord. In unc-3 mutants, many active zone puncta are clustered and SYD-2::GFP is diffusely localized along the axon.
SYD-2::GFP fusion protein is localized to discrete puncta of 200-400 nm in diameter along the dorsal and ventral nerve cord. In unc-3 mutants, many active zone puncta are clustered and SYD-2::GFP is diffusely localized along the axon.