"To examine the effect of increased dosage of
pdp-1, we generated transgenic worms bearing a translational fusion containing
pdp-1 fused to gfp and driven by its own promoter (
pdp-1::gfp). In addition, we also crossed the
pdp-1::gfp worms to
daf-2(
e1370) mutants to generate the
daf-2(
e1370);
pdp-1::gfp strain. Overexpression of
pdp-1 results in a significant extension in lifespan compared to wild-type worms (Figure 2D and Figure S4). Interestingly,
pdp-1 overexpression further extends the lifespan of
daf-2(
e1370) mutants (Figure 2B and Figure S4). In both of these cases, the increased lifespan was suppressed by
daf-16 RNAi (Figure S5)."