Under unstressed conditions, attenuating hcf-1 function increased the nuclear accumulation of SKN-1 protein. Interestingly, the amount of elevated intestinal nuclear SKN-1 was even further augmented in hcf-1 mutants upon exposure to NaAs and t-BOOH.
"Worms were treated for 6 h on 51 mMNaCl agar plates containing either 1 mg/ml kasugamycin or 1 mg/ml cycloheximide. Kasugamycin inhibits translation initiation while cycloheximide blocks the elongation step of protein synthesis. As shown in Fig. 1E, both compounds elevated gpdh-1 mRNA levels 3-16 fold (P < 0.005)."