In most control animals, there were 12 VU cells expressing egl-13::gfp. In egl-43i animals, the number of egl-13::gfp-positive cells was reduced to fewer than four gfp-expressing VU cells.
In most control animals, there were 12 VU cells expressing lin-11::gfp. In egl-43i animals, the number of lin-11::gfp-positive cells was reduced to fewer than four gfp-expressing VU cells.
Suppression of the anchor cell deficient phenotype observed in activated LIN-12 (lin-12(n137)), Thus, egl-43 acts downstream of or in parallel to lin-12 during AC/VU specification.
In fos-1(ar105) homozygous L3 larvae carrying the 1.7-Lp7gfp reporter, no GFP expression could be detected in the AC, and GFP expression in the VU cells was also strongly reduced.
fos-1 RNAi eliminated egl-43::YFP expression in the DU and VU descendants as well as in the AC, suggesting that fos-1 is an upstream regulator of egl-43 in these gonadal cells.