lin-35 : pha-4 : ubc-18

"An examination of wild-type embryos at the 1.5-fold stage identified an average of 1003 (n=11; range, 95-104) GFP+ cells in the head region. This figure is in close agreement with previously reported numbers using antibodies against PHA-4 in similar-stage embryos (973) (Horner et al., 1998) (Fig. 3A,B). An analysis of Pun double-mutant [ubc-18; lin-35(RNAi)] embryos revealed an average of 9313 GFP+ cells (n=13) with numbers ranging from 66-105. Importantly, 8/13 mutant embryos contained 95 or more GFP+ cells, whereas only 3/13 contained fewer than 91 cells (Fig. 3C,D)... Similar results were also obtained by examining pha-4::GFP-expressing cells in L1 larvae of double-mutant and wild-type animals (Fig. 3E-H; data not shown)."

lin-35 : pha-4 : ubc-18

"An examination of wild-type embryos at the 1.5-fold stage identified an average of 1003 (n=11; range, 95-104) GFP+ cells in the head region. This figure is in close agreement with previously reported numbers using antibodies against PHA-4 in similar-stage embryos (973) (Horner et al., 1998) (Fig. 3A,B). An analysis of Pun double-mutant [ubc-18; lin-35(RNAi)] embryos revealed an average of 9313 GFP+ cells (n=13) with numbers ranging from 66-105. Importantly, 8/13 mutant embryos contained 95 or more GFP+ cells, whereas only 3/13 contained fewer than 91 cells (Fig. 3C,D)... Similar results were also obtained by examining pha-4::GFP-expressing cells in L1 larvae of double-mutant and wild-type animals (Fig. 3E-H; data not shown)."