When three E3 ubiquitin ligases (marc-6, hrd-1/sel-11, and hrdl-1) were simultaneously perturbed by mutation or RNAi, worms exhibited a significant synthetic slow growth phenotype.
In wild type, lin-32p::mRFP::unc-60A 3'UTR was intensely detected in every R(n).a + p. However, in plx-1 mutants, R(1).a + p, R(2).a + p and R(5).a + units displayed pronounced decreases in expression of mRFP.
In wild type, lin-32p::mRFP::unc-60A 3'UTR was intensely detected in every R(n).a + p. However, in smp-1smp-2 mutants, R(1).a + p, R(2).a + p and R(5).a + units displayed pronounced decreases in expression of mRFP.
Expression of mec-2-lacZ in the ALML/R, AVM, and PVM touch cells was greatly decreased (<5% of the cells stained); 67% of the PLML/R cells still stained in mec-3(u6).