acacetin : gst-4

Acacetin (25 uM) significantly enhanced the expression level GST-4 in comparison to untreated control.

acacetin : sod-3

Acacetin (25 uM) significantly enhanced the expression level of SOD-3 in comparison to untreated control.

Sodium Selenite : sod-3

Selenite (0.01 uM) upregulated expressions of transgenic C. elegans carrying sod-3::green fluorescent protein (GFP) and hsp-16.2::GFP.

mtl-1 : zinc

In the absence of supplemental zinc, transgenic animals displayed low level, variable mtl-1::GFP expression in the pharynx and no detectable GFP expression in intestinal cells. By contrast, in the presence of 200 uM supplemental zinc, worms displayed strong GFP expression in intestinal cells, whereas pharyngeal expression levels were not altered. Quantitative analysis using qRT-PCR showed that GFP mRNA levels were increased ~70-fold in response to 200 uM supplemental zinc compared to 0 uM supplemental zinc.

Sodium Selenite : hsp-16.2

Selenite (0.01 uM) upregulated expressions of transgenic C. elegans carrying sod-3::green fluorescent protein (GFP) and hsp-16.2::GFP.

mtl-1 : zinc

In the absence of supplemental zinc, transgenic animals displayed low level, variable mtl-1::GFP expression in the pharynx and no detectable GFP expression in intestinal cells. By contrast, in the presence of 200 uM supplemental zinc, worms displayed strong GFP expression in intestinal cells, whereas pharyngeal expression levels were not altered. Quantitative analysis using qRT-PCR showed that GFP mRNA levels were increased ~70-fold in response to 200 uM supplemental zinc compared to 0 uM supplemental zinc.

aex-1 : aex-5 : unc-13

"The aex-5(sa23) mutant showed a diffuse UNC-13::GFP distribution: UNC-13::GFP density was 0.6 puncta/10 um compared to 1.7 puncta/10 um in the wild-type (Figure 8). Muscle-specific expression of [Pmyo-3::AEX-5(+)] rescued UNC-13 localization in the ventral nerve cord, but neural/intestinal-specific expression did not (Figure 8)."

daf-16 : juglone : juglone, a generator of reactive oxyger species (ROS)

Juglone at a concentration of 40 uM induced a nuclear accumulation of DAF-16 in TJ356 animals expressing a DAF-16::GFP fusion protein.

aex-1 : aex-5 : unc-13

"The aex-5(sa23) mutant showed a diffuse UNC-13::GFP distribution: UNC-13::GFP density was 0.6 puncta/10 um compared to 1.7 puncta/10 um in the wild-type (Figure 8). Muscle-specific expression of [Pmyo-3::AEX-5(+)] rescued UNC-13 localization in the ventral nerve cord, but neural/intestinal-specific expression did not (Figure 8)."

aex-1 : aex-5 : unc-13

"The aex-5(sa23) mutant showed a diffuse UNC-13::GFP distribution: UNC-13::GFP density was 0.6 puncta/10 um compared to 1.7 puncta/10 um in the wild-type (Figure 8). Muscle-specific expression of [Pmyo-3::AEX-5(+)] rescued UNC-13 localization in the ventral nerve cord, but neural/intestinal-specific expression did not (Figure 8)."