"To determine whether class I
unc-52 mutations disrupted UNC-5 function, they were passed into a strain containing evIs99, an integrated
emb-9::
unc-5 transgenic array. In contrast to an
unc-6 mutation (Su et al., 2000),
unc-52(
e1421) did not completely block precocious ventral-to-dorsal migrations (Fig. 3C). In evIs99 alone, precocious ventral-to-dorsal migrations occurred in 21% of anterior and 39% of posterior DTCs (n = 206). In the evls99;
unc-52(
e1421) background, these precocious migrations occurred in 11% (19/166; P < 0.005) of anterior and 38% (50/166; P > 0.4) of posterior DTCs. The frequency of these precocious migrations was significantly reduced relative to evIs99 alone only for the anterior DTCs."