Mn uptake was increased due to deletion of djr1.1, whereas the elemental distribution of Mn was altered significantly in the djr1.1, djr1.2 double deletion mutants.
Mutants containing a-Syn accumulated less Mn compared to the deletion mutant alone, the decrease in accumulation was significant at 7.5 and 10 mM Mn for the djr1.1 mutants.
One-way ANOVA analysis showed a significantly increased sensitivity of the a-Syn-containing djr1.1 mutants towards Mn compared to the djr1.1 mutants alone
Using Real Time RT PCR, authors show that pdr1 mutants have inherently higher dat-1 mRNA levels, whereas the expression is reduced in both Mn treated and untreated djr1.1 mutants compared to WT worms.
Mechanosensory termini of the CEP and ADE dopaminergic neurons in dat-1::GFP expressing worms display discontinuous GFP staining 24 h after 50 mM Mn acute exposure at L1, revealing their degeneration.
Young nematodes exposed to MnCl2 for 30 min show a 3-fold increase in GST-1 mRNA levels. Exposure to Mn results in a 3-4-fold increase in GST-1 protein levels.