Exogenous DA [(25S),26-3-
keto-4-cholestenoic acid] added to the medium at the indicated concentration can rescue the Daf-c phenotype of
daf-9(
dh6). This rescue is more efficient if
hbl-1 activity is reduced. However,
hbl-1 is unable to affect dauer formation at very low or very high DA concentrations. Also, reducing
hbl-1 activity in a
daf-9(
dh6) background has no effect on the gonad migration phenotype observed in these animals at limiting concentrations of DA.