"Under standard uncrowded culture conditions with abundant food, DAF-16::GFP is not localized (see Figures 1d,e, 3a1-
a4, and 4a). However, under starvation conditions, DAF-16::GFP becomes progressively nuclear localized (see Figure 3b1-
b3). Animals placed in the same conditions with food did not exhibit nuclear localization, even after many hours (Figure 3a1-
a4)."