clr-1 : vpr-1

"An MSP Receptor Screen Identifies SAX-3 Robo and CLR-1 Lar-Like Receptors."

sax-3 : vpr-1

"An MSP Receptor Screen Identifies SAX-3 Robo and CLR-1 Lar-Like Receptors."

Interaction involving msp-142

"the carboxy-terminal beta strands are directly involved in interactions required for MSP filament assembly."

Interaction involving msp-142

"the carboxy-terminal beta strands are directly involved in interactions required for MSP filament assembly."

alg-3 : alg-4 : gsp-3

"Unlike WT spermatozoa, however, these alg-3/4 spermatozoa never formed pseudopods of normal morphology or polarity, with MSP and GSP-3 localized to the leading edge of the pseudopod (Figure 3B). Instead, we observed that (1) MSP and GSP-3 remained in large punctae that resemble sperm organelles called fibrousbodies (FBs) (5%), (2) MSP localized to the cortex and GSP-3 to the cytoplasm of the spermatid but lacked polarity (3%), or (3) GSP-3 remained in the cytoplasm and spermatids formed a multibranched, spikey pseudopod containing MSP (4%)."

ire-1 : msp-76

msp-76 RNAi in the ire-1(v33) mutant background resulted in a synthetic severe growth defect

apr-1 : sys-1

GFP::SYS-1 asymmetry between MSa/MSp and Ea/Ep pairs of sisters was abolished or defective in apr-1(RNAi) embryos.

alg-3 : alg-4 : gsp-3

"we used immunofluorescence (IF) assays to detect MSP and GSP-3 proteins and fluorescence in situ hybridization (FISH) to detect msp transcripts. Interestingly, IF of MSP and GSP-3, two positively regulated targets, suggested that these proteins were only slightly reduced in alg-3/4 germlines during spermatogenesis and in haploid spermatids at 20C compared to WT (Figure 3A). At 25C, however, MSP and GSP-3 were severely reduced throughout the spermatogenic germline of alg-3/4 males compared toWTand failed to accumulate in spermatids (Figure 3A). Although GSP-3 protein was depleted overall, we found that it also accumulated abnormally in the nuclei of alg-3/4 mutants, particularly at 25C (Figure 3A)."

mom-2 : sys-1

GFP::SYS-1 asymmetry between MSa/MSp and Ea/Ep pairs of sisters was abolished or defective in most mom-2(or42) embryos.

fog-3 : msp-3

"P-granule, pgl-1;pgl-3 and csr-1 RNAi was performed in ife-1(bn127) mutants, alg-4(ok1041); alg-3(tm1155) double mutants, and fog-3(q470) loss-of-function heterozygous mutants (supplementary material Fig. S6; Table 1). ife-1(bn127) single mutants and alg-4(ok1041); alg-3(tm1155) double mutants did not suppress msp-3 expansion upon csr-1, P-granule and pgl-1; pgl-3 depletion. Similarly, msp-3 expansion was not suppressed in fog-3(q470/+) heterozygotes. However, msp-3 transcripts are absent in fog-3(q470) homozygotes."