lin-3 : sys-1

"we used a lin-3::lacZ marker, which highlights anchor cells (Hill and Sternberg, 1992). Among sys-1 mutant hermaphrodite L3s, most (13/22) had two adjacent anchor cells (Fig. 7). Others possessed either one anchor cell as normal (5/22) or three (4/22)."

cki-1 : lin-3

"Wild-type hermaphrodites have one anchor cell that can be detected using a lin-3::lacZ or cdh-3::GFP reporter transgene (Hill and Sternberg, 1992; Pettitt et al., 1996). cki-1(RNAi) animals display more than one anchor cell since multiple cells stain for lin-3::lacZ and show cdh-3::GFP expression (Fig. 1E; data not shown)."

daf-11 : trx-1

At 25 deg C, but enhances the Daf-C at 15 deg C.

daf-11 : trx-1

At 15 deg C but suppresses the Daf-C phenotype of sa195 at 25 deg C.

acdh-11 : high temperature

Growth at 25 C as opposed to 20 C or 15 C caused marked up regulation of Pacdh-11::GFP predominantly in the intestine.

daf-2 : trx-1

No animals were dauer at 15 deg C and no enhancement or suppression of the Daf-C phenotype was observed at 25 deg C.

daf-2 : trx-1

No animals were dauer at 15 deg C and no enhancement or suppression of the Daf-C phenotype was observed at 25 deg C.

daf-2 : trx-1

No animals were dauer at 15 deg C and no enhancement or suppression of the Daf-C phenotype was observed at 20 or 25 deg C.

lit-1 : mom-2

100% of embryos lack endoderm in double mutants at 15 deg C and 25 deg C, whereas lit-1 mutants only exhibit this phenotype at 25 deg C and mom-2 mutants exhibit this phenotype only partially at 22 deg C.

gip-1 : tbg-1

"In wild-type embryos, CeGrip-1 colocalizes with c-tubulin at centrosomes during interphase (unpublished data) and mitosis (Fig. 4 C, left). In gip-1(RNAi) embryos, c-tubulin fails to target to centrosomes (n = 10 embryos; Fig. 4 C, right)"