mec-15 : mec-7

"This mec-7(u278) suppressor screen (see Materials and Methods) yielded a mec-15 nonsense allele u1042(R26*) that completely suppressed the growth of ALM-PN in mec-7(u278) mutants. Another mec-15 loss-of-function (lf) allele u75(Q118*) showed similar suppression (Fig. 1A,B; Fig. 2A)." ALM-PN = ALM-posterior neurite growth phenotype

arx-2 : rab-5

"In arx-2 RNAi-treated embryos, it also appeared that RAB-5-positive foci were enlarged, similar to our observation in the GFP-EEA-1(2XFYVE)-expressing strain. To determine whether this was the case, we measured the area of RAB-5-labeled endosomes at eight time points from PN meeting through 8:00 after PN meeting. The average areas of the endosomes in controls were initially a bit larger earlier in the cell cycle and then decreased slightly, ranging from 4.6 to 3.7 μm² over these time points (Figure 3F). In arx-2 RNAi-treated embryos, the average area was similar to that in controls at PN meeting and at 1:00 after PN meeting (Figure 3F). However, from 2:00 to 5:00 after PN meeting, the average endosome areas were significantly larger than in the controls, >5.0 μm², suggesting that ARX-2 affects regulation of early endosome size and possibly trafficking associated with the early endosome."

mec-15 : mec-7

"This mec-7(u278) suppressor screen (see Materials and Methods) yielded a mec-15 nonsense allele u1042(R26*) that completely suppressed the growth of ALM-PN in mec-7(u278) mutants. Another mec-15 loss-of-function (lf) allele u75(Q118*) showed similar suppression (Fig. 1A,B; Fig. 2A)." ALM-PN = ALM-posterior neurite growth phenotype

arx-2 : par-6

In the arx-2 RNAi-treated embryos, the cortical area occupied by GFP-PAR-6 was somewhat reduced during maintenance phase, however, this was statistically significant only at the PN meeting+1:00 min time point, further suggesting that disruption of Arp2/3 leads to a destablization of PAR asymmetry specifically during maintenance phase.

arx-2 : cdc-42

In arx-2 RNAi-treated embryos, the GFP-CDC-42-labeled structures during establishment phase are shorter and more condensed than in controls, averaging 1.761.05 m in length. CDC-42 still becomes enriched at the anterior end of the embryo and is maintained. In contrast to the controls, we observed little change in the length of the foci between establishment and maintenance phase in arx-2 RNAi-treated embryos (1.631.01 m in length). In terms of the cortical area, CDC-42 occupies a greater area of the cortex 4:00 min after PN meeting than in the controls (wild-type/control n=7 embryos; arx-2 RNAi n=7 embryos), indicating that CDC-42 dynamics are significantly disrupted.