"In wild-type males, the distal Z1/Z4 daughters become DTCs, which are tiny and express
lag-2::GFP weakly (Fig. 5A, B)... We found that most
pop-1(Sys) males made a robust germline, indicating that at least one DTC was made (Table 2). The production of DTCs in
pop-1(Sys) males was corroborated by
lag-2::GFP (Table 2), an assay that permits direct visualization of DTCs, but suffers from faint staining and an apparent enhancement of the Glp phenotype by the transgene (Table 2). Nonetheless, we found that 50% of
pop-1(Sys) males made two DTCs (not shown). The wild-type male linker cell expresses
lag-2::GFP intensely (Fig. 5A,B) (Blelloch et al., 1999). Similarly, all
pop-1(Sys) males had at least one, and
pop-1(
q645) males sometimes had two LCs (Table 2; Fig. 5C,D)."