Authors examined the expression level of mannosidase II, a glycosidase localized in the medial Golgi stack using pwIs503[
vha-6p::mans::gfp] and found it was severely reduced. pwIs503 contains a signal sequence and a Golgi membrane-anchor domain of C. elegans alpha-mannosidase II (
aman-2) fused to GFP, driven by the
vha-6 promoter.