In wild-type, RME-2-GFP localizes to cortical endosomes and the plasma membrane, whereas RME-2-GFP accumulates in deep cytoplasmic vesicles in the oocytes of snap-29(RNAi) animals.
"We focused on two stages, Day 0 young adults and Day 8 old adults in order to rank changes in expression of mature miRNAs during aging... We confirmed these results by biological replicates of the deep-sequencing libraries for Day 0 and Day 8, using independently prepared RNA samples, and also by quantitative RT-PCR (qRT-PCR) (Fig. 2B; Supplemental Fig. S2), supporting the reliability of our data."
"We focused on two stages, Day 0 young adults and Day 8 old adults in order to rank changes in expression of mature miRNAs during aging... We confirmed these results by biological replicates of the deep-sequencing libraries for Day 0 and Day 8, using independently prepared RNA samples, and also by quantitative RT-PCR (qRT-PCR) (Fig. 2B; Supplemental Fig. S2), supporting the reliability of our data."
"We focused on two stages, Day 0 young adults and Day 8 old adults in order to rank changes in expression of mature miRNAs during aging... We confirmed these results by biological replicates of the deep-sequencing libraries for Day 0 and Day 8, using independently prepared RNA samples, and also by quantitative RT-PCR (qRT-PCR) (Fig. 2B; Supplemental Fig. S2), supporting the reliability of our data."
"We focused on two stages, Day 0 young adults and Day 8 old adults in order to rank changes in expression of mature miRNAs during aging... We confirmed these results by biological replicates of the deep-sequencing libraries for Day 0 and Day 8, using independently prepared RNA samples, and also by quantitative RT-PCR (qRT-PCR) (Fig. 2B; Supplemental Fig. S2), supporting the reliability of our data."