fat-5 : fat-7 : irg-1 : sams-1

"We validated expression profiling results by quantitative RT-PCR of selected genes normalized to act-1 levels after sams-1(RNAi) (not shown), as well as in sams-1(ok3033) animals (Figures S1B and S1C). We also normalized qPCR validations to an external "spiked-in" RNA (JNK1a1 alpha, gift of Dr. Roger Davis). These results were similar to the microarray and to the act-1 normalized qPCR for upregulated (Figures 1C and 1D), downregulated (Figure 1E), or unchanged genes (Figure 1F) tested."

choline : cyp-13A5 : hpo-6 : irg-1 : irg-2 : vhp-1

"We validated expression profiling results by quantitative RT-PCR of selected genes normalized to act-1 levels after sams-1(RNAi) (not shown), as well as in sams-1(ok3033) animals (Figures S1B and S1C). We also normalized qPCR validations to an external "spiked-in" RNA (JNK1a1 alpha, gift of Dr. Roger Davis). These results were similar to the microarray and to the act-1 normalized qPCR for upregulated (Figures 1C and 1D), downregulated (Figure 1E), or unchanged genes (Figure 1F) tested."

cyp-13A5 : hpo-6 : irg-1 : irg-2 : sams-1 : vhp-1

"We validated expression profiling results by quantitative RT-PCR of selected genes normalized to act-1 levels after sams-1(RNAi) (not shown), as well as in sams-1(ok3033) animals (Figures S1B and S1C). We also normalized qPCR validations to an external "spiked-in" RNA (JNK1a1 alpha, gift of Dr. Roger Davis). These results were similar to the microarray and to the act-1 normalized qPCR for upregulated (Figures 1C and 1D), downregulated (Figure 1E), or unchanged genes (Figure 1F) tested."

sams-1 : warf-1

"We validated expression profiling results by quantitative RT-PCR of selected genes normalized to act-1 levels after sams-1(RNAi) (not shown), as well as in sams-1(ok3033) animals (Figures S1B and S1C). We also normalized qPCR validations to an external "spiked-in" RNA (JNK1a1 alpha, gift of Dr. Roger Davis). These results were similar to the microarray and to the act-1 normalized qPCR for upregulated (Figures 1C and 1D), downregulated (Figure 1E), or unchanged genes (Figure 1F) tested."

fat-5 : fat-7 : sams-1 : warf-1

"We validated expression profiling results by quantitative RT-PCR of selected genes normalized to act-1 levels after sams-1(RNAi) (not shown), as well as in sams-1(ok3033) animals (Figures S1B and S1C). We also normalized qPCR validations to an external "spiked-in" RNA (JNK1a1 alpha, gift of Dr. Roger Davis). These results were similar to the microarray and to the act-1 normalized qPCR for upregulated (Figures 1C and 1D), downregulated (Figure 1E), or unchanged genes (Figure 1F) tested."

sams-1 : vit-1 : vit-3

"We validated expression profiling results by quantitative RT-PCR of selected genes normalized to act-1 levels after sams-1(RNAi) (not shown), as well as in sams-1(ok3033) animals (Figures S1B and S1C). We also normalized qPCR validations to an external "spiked-in" RNA (JNK1a1 alpha, gift of Dr. Roger Davis). These results were similar to the microarray and to the act-1 normalized qPCR for upregulated (Figures 1C and 1D), downregulated (Figure 1E), or unchanged genes (Figure 1F) tested."

act-1 : ama-1 : rpb-2 : sams-1 : taf-1

"We validated expression profiling results by quantitative RT-PCR of selected genes normalized to act-1 levels after sams-1(RNAi) (not shown), as well as in sams-1(ok3033) animals (Figures S1B and S1C). We also normalized qPCR validations to an external "spiked-in" RNA (JNK1a1 alpha, gift of Dr. Roger Davis). These results were similar to the microarray and to the act-1 normalized qPCR for upregulated (Figures 1C and 1D), downregulated (Figure 1E), or unchanged genes (Figure 1F) tested."

choline : fat-5 : fat-7 : warf-1

"We validated expression profiling results by quantitative RT-PCR of selected genes normalized to act-1 levels after sams-1(RNAi) (not shown), as well as in sams-1(ok3033) animals (Figures S1B and S1C). We also normalized qPCR validations to an external "spiked-in" RNA (JNK1a1 alpha, gift of Dr. Roger Davis). These results were similar to the microarray and to the act-1 normalized qPCR for upregulated (Figures 1C and 1D), downregulated (Figure 1E), or unchanged genes (Figure 1F) tested."