"In unc-104 animals, GFP-tagged synaptobrevin (Nonet, 1999; and Figure 8B) and other synaptic vesicle components were restricted to cell bodies (Nonet et al., 1993, 1997, 1998), and fluorescence was distributed within the intracellular space."
Exposure to the dithiocarbamate organometallic fungicide macozeb induced expression of the reporter [hsp-16p::lacZ] in adult C. elegans. A range of dosages from 50-500 g/mL were tested. The effect was observed at all dosages, but the highest at 500 g/mL. See Figures 1 and 2.
Exposure to the dithiocarbamate organometallic fungicide maneb induced expression of the reporter [hsp-16p::lacZ] in adult C. elegans. A range of dosages from 50-500 g/mL were tested. The effect was observed at all dosages, but the highest at 500 g/mL. See Figures 1 and 2.
"Worms were treated for 6 h on 51 mMNaCl agar plates containing either 1 mg/ml kasugamycin or 1 mg/ml cycloheximide. Kasugamycin inhibits translation initiation while cycloheximide blocks the elongation step of protein synthesis. As shown in Fig. 1E, both compounds elevated gpdh-1 mRNA levels 3-16 fold (P < 0.005)."
"Worms were treated for 6 h on 51 mMNaCl agar plates containing either 1 mg/ml kasugamycin or 1 mg/ml cycloheximide. Kasugamycin inhibits translation initiation while cycloheximide blocks the elongation step of protein synthesis. As shown in Fig. 1E, both compounds elevated gpdh-1 mRNA levels 3-16 fold (P < 0.005)."
Treatment with BIBU1361 showed that the lower concentration 0.1 mg/ml induced beta-Galactosidase expression first in the intestinal cells of adult PC72 C. elegans. At higher concentrations (e.g., 0.4 mg/ml BIBU1361), a strong expression of h-Galactosidase was observed in cells of the head pharynx region and the midbody region.
Transgenic PC72 nematodes exposed to the chemical analogue BIBU1476 displayed a weak beta-Galactosidase expression at the concentration 0.1 mg/ml. At the higher concentrations (e.g., 0.4 mg/ml BIBU1476), the animals showed minor levels of stress responses, and in comparison to BIBU1361 and BIBX1382, the expression of beta-Galactosidase was generally very low.