dlk-1 : rpm-1

The RPM-1 RING finger (RF) domain stimulates DLK-1 ubiquitination.

dlk-1 : rpm-1

The RPM-1 RING finger (RF) domain stimulates DLK-1 ubiquitination.

age-1 : lin-35

age-1 reduction-of-function (rf) mutations were able to partially suppress the starvation survival defects associated with lin-35/Rb(lf).

itr-1 : vav-1

itr-1(gf) suppresses reduced brood size significantly. itr-1(rf) enhances the reduced brood size phenotype. These double mutants are almost completely sterile. let-23(gf) does not have much effect.

ain-1 : ain-2

We did not observe significant reduction of mir-48 and mir-84 levels in ain-1(lf);ain-2(rf) animals. Moreover, there was no accumulation of miRNA precursors in the double mutant.

itr-1 : vav-1

itr-1(gf) suppresses reduced brood size significantly. itr-1(rf) enhances the reduced brood size phenotype. These double mutants are almost completely sterile. let-23(gf) does not have much effect.

let-60 : lin-1

"In 72% of lin-15(rf) (n = 18) and 68% of let-60(gf) animals (n = 34), LIP-1::GFP was up-regulated in P3.p, P4.p, or P8.p in addition to P5.p and P7.p (Fig. 2F)."

ceh-13 : lin-1

A loss of ceh-13::GFP expression in B in lin-1(rf), indicating that there is a positive requirement for LIN-1 for ceh-13/Hox1 expression in B. Furthermore, a loss of ceh-13::GFP expression in B in lin-1(gf) mutants was observed, suggesting that LIN-1 has a negative effect on ceh-13/Hox1 expression in B. In addition, ceh-13::GFP was ectopically expressed in B in lin-1(rf) males, which suggests that LIN-1 inhibits B from expressing the B fate. Therefore, LIN-1 positively and negatively regulates transcription of ceh-13.

ceh-10 : ceh-17

"We then scored the extent of ALA axon migration and found that the ceh-10(rf) animals showed a ceh-17-like truncation of the ALA axons, albeit less severe (Fig 6B,C). While the ceh-17(lf) axon migration phenotype is similar when scored in either L1 or L2 stage animals, we found that a greater fraction of ceh-10(rf) ALA axons reach the tail by the L2 stage than at L1. This was surprising, as wild-type ALA axons complete their migration before hatching (Pujol et_al, 2000). To determine if the postembryonic ALA axon migration seen in ceh-10(rf) mutant animals might be dependent on CEH-17 activity, we examined ALA axon migration in ceh-17(np1);ceh-10(ct78) animals. We found the extent of axon migration to be similar between the L1 and the L2 stages, and more severe at each stage than in either single mutant (Fig 6C)."

ceh-13 : lin-1

A loss of ceh-13::GFP expression in B in lin-1(rf), indicating that there is a positive requirement for LIN-1 for ceh-13/Hox1 expression in B. Furthermore, a loss of ceh-13::GFP expression in B in lin-1(gf) mutants was observed, suggesting that LIN-1 has a negative effect on ceh-13/Hox1 expression in B. In addition, ceh-13::GFP was ectopically expressed in B in lin-1(rf) males, which suggests that LIN-1 inhibits B from expressing the B fate. Therefore, LIN-1 positively and negatively regulates transcription of ceh-13.