"Depletion of CeBUB-1, HCP-1, or CeCLASP-2 had no effect on the kinetochore targeting of KNL-1 (Fig. 6E). Because HCP-1 is functionally redundant with HCP-2 (Moore et al. 1999), the reverse RNAi was performed using a mixture of HCP-1/HCP-2 double-stranded RNAs, and depletion was assessed using a mixture of anti-HCP-1 and anti-HCP-2 antibodies."
"Depletion of CeBUB-1, HCP-1, or CeCLASP-2 had no effect on the kinetochore targeting of KNL-1 (Fig. 6E). Because HCP-1 is functionally redundant with HCP-2 (Moore et al. 1999), the reverse RNAi was performed using a mixture of HCP-1/HCP-2 double-stranded RNAs, and depletion was assessed using a mixture of anti-HCP-1 and anti-HCP-2 antibodies."
These animals showed no hlh-8::gfp expression throughout the M lineage and lacked all M-derived CCs. This is an enhancement of the defects in the M-lineage compared to either RNAi or mutant alone.
These animals showed no hlh-8::gfp expression throughout the M lineage and lacked all M-derived CCs. This is an enhancement of the defects in the M-lineage compared to either RNAi or mutant alone.