"Depletion of CeBUB-1, HCP-1, or CeCLASP-2 had no effect on the kinetochore targeting of KNL-1 (Fig. 6E). Because HCP-1 is functionally redundant with HCP-2 (Moore et al. 1999), the reverse RNAi was performed using a mixture of HCP-1/HCP-2 double-stranded RNAs, and depletion was assessed using a mixture of anti-HCP-1 and anti-HCP-2 antibodies."
"Depletion of CeBUB-1, HCP-1, or CeCLASP-2 had no effect on the kinetochore targeting of KNL-1 (Fig. 6E). Because HCP-1 is functionally redundant with HCP-2 (Moore et al. 1999), the reverse RNAi was performed using a mixture of HCP-1/HCP-2 double-stranded RNAs, and depletion was assessed using a mixture of anti-HCP-1 and anti-HCP-2 antibodies."
100% of embryos lack endoderm in double mutants at 15 deg C and 25 deg C, whereas lit-1 mutants only exhibit this phenotype at 25 deg C and mom-2 mutants exhibit this phenotype only partially at 22 deg C.
"In wild-type embryos, CeGrip-1 colocalizes with c-tubulin at centrosomes during interphase (unpublished data) and mitosis (Fig. 4 C, left). In gip-1(RNAi) embryos, c-tubulin fails to target to centrosomes (n = 10 embryos; Fig. 4 C, right)"