After mild heat-shock treatment at the L2-larval stage, the GFP signals were sharply increased in 50 mM or 100 mM of Pb exposed Pmtl-1::GFP and Pmtl-2::GFP transgenic adult animals compared with those treated with mild heat-shock or Pb exposure alone.
After mild heat-shock treatment at the L2-larval stage, the GFP signals were sharply increased in 50 mM or 100 mM of Pb exposed Pmtl-1::GFP and Pmtl-2::GFP transgenic adult animals compared with those treated with mild heat-shock or Pb exposure alone.
"Depletion of CeBUB-1, HCP-1, or CeCLASP-2 had no effect on the kinetochore targeting of KNL-1 (Fig. 6E). Because HCP-1 is functionally redundant with HCP-2 (Moore et al. 1999), the reverse RNAi was performed using a mixture of HCP-1/HCP-2 double-stranded RNAs, and depletion was assessed using a mixture of anti-HCP-1 and anti-HCP-2 antibodies."
"Depletion of CeBUB-1, HCP-1, or CeCLASP-2 had no effect on the kinetochore targeting of KNL-1 (Fig. 6E). Because HCP-1 is functionally redundant with HCP-2 (Moore et al. 1999), the reverse RNAi was performed using a mixture of HCP-1/HCP-2 double-stranded RNAs, and depletion was assessed using a mixture of anti-HCP-1 and anti-HCP-2 antibodies."
The percentage of the population with hsp-16.2::gfp expression was increased by 28.6%, and 52.4%, respectively, in Pb-exposed nematodes at concentrations of 2.5 and 50 mol/L.