mir-1018 : mir-1022 : mir-1817 : mir-1829.2 : mir-1833 : mir-2209.1 : mir-2210 : mir-2215 : mir-2218.1 : mir-2218.2 : mir-2220 : mir-241 : mir-242 : mir-243 : mir-255 : mir-258.1 : mir-38 : mir-47 : mir-49 : mir-54 : mir-76 : mir-785 : mir-799 : mir-80 : mir-800 : mir-90 : nicotine

"Three miRNAs were altered in response to the lower nicotine concentration (20uM). Of them, miR-80 and miR-79 were upregulated by 1.0 (P=0.045) and 0.9 (P=0.022) fold, respectively. Conversely, miR-230 was inhibited by 0.5 fold (P=0.019). On the other hand, the expression of 38 miRNAs changed with statistical significance in worms treated with high nicotine concentration (20 mM). The fold changes and P-values are shown in Table 1. About 78% of the altered miRNAs were upregulated with fold changes ranging from 0.5 to 3.4 fold. The most upregulated miRNAs were miR-2220 with 3.4-fold change (P=0.034) followed by mir-90 (P=0.045) and mir- 2210 (P=0.03) with >1.5 folds change. Other miRNAs, such as mir-47 (P=0.027), mir-2216 (P=0.034), mir-49 (P=0.019), mir-38 (P=0.004), mir-255 (P=0.003), mir-1829 (P=0.037), mir-785 (P=0.003), and mir-241 (P=0.017), were upregulated by at least 100%. The remaining 22% miRNAs that were affected by the 20mM nicotine treatment were downregulated by at least 50%. The most downregulated miRNAs were mir-58 (P=0.014) followed by mir-1821 (P=0.03) and lin-4 (P=0.002) (Table 1). In addition, miR-80 was the only miRNA upregulated in both low and high concentration nicotine treatments (Fig. 2)."

aging : mir-2 : mir-2209.1 : mir-2209.2 : mir-2209.3 : mir-43 : mir-54 : mir-74 : mir-794 : mir-796 : mir-87

"We focused on two stages, Day 0 young adults and Day 8 old adults in order to rank changes in expression of mature miRNAs during aging... We confirmed these results by biological replicates of the deep-sequencing libraries for Day 0 and Day 8, using independently prepared RNA samples, and also by quantitative RT-PCR (qRT-PCR) (Fig. 2B; Supplemental Fig. S2), supporting the reliability of our data."

aging : mir-240 : mir-34 : mir-35 : mir-36 : mir-37 : mir-38 : mir-39 : mir-40 : mir-41 : mir-786

"We validated the altered expression of age-associated miRNAs using transgenic animals carrying constructs of the miRNA promoter fused to a GFP marker gene. Transgenic lines used in this study contained transgenes integrated into chromosomes and were crossed into the spe-9(hc88) mutant background. We tested changes in GFP signal intensity during aging at Day 0, Day 3, and Day 6 of adulthood... For example, mir-34, which showed a dramatic increase in expression of the mature miRNA during aging, exhibited a similar increase in GFP signals in aged transgenic animals (Fig. 5)... Interestingly, in many cases of miRNAs up-regulated with age, the GFP signals seem to be expressed in tissues or cells in which they are not expressed at earlier stages. The GFP signals from the mir-34Tgfp transgenic line were detectable de novo in cells on the ventral side in most aging animals (Fig. 5; marked by arrowheads in larger images in Supplemental Fig. S6B)."

mir-58.1 : mir-80 : mir-81 : mir-82

"Neither small RNA profiling nor miRNA qRT-PCR identified significant changes in the expression of miRNAs other than those that were deleted in the mutant backgrounds (Fig. 4A,B)"

mir-58.1 : mir-80 : mir-81 : mir-82

"Neither small RNA profiling nor miRNA qRT-PCR identified significant changes in the expression of miRNAs other than those that were deleted in the mutant backgrounds (Fig. 4A,B)"

mir-58.1 : mir-80 : mir-81 : mir-82

"Neither small RNA profiling nor miRNA qRT-PCR identified significant changes in the expression of miRNAs other than those that were deleted in the mutant backgrounds (Fig. 4A,B)"

mir-58.1 : mir-80 : mir-81 : mir-82

"Neither small RNA profiling nor miRNA qRT-PCR identified significant changes in the expression of miRNAs other than those that were deleted in the mutant backgrounds (Fig. 4A,B)"

aging : mir-235 : mir-53 : mir-58.1 : mir-58.2 : mir-64 : mir-65 : mir-80 : mir-81

"To examine changes with age in animals lacking a germline, we collected individual glp-1 mutants spanning 4 adult ages that included both young and old animals (Figure 3A). Interestingly we found that expression from the mir-35-41 gene cluster was nearly absent in animals that lacked a gonad. Of the mir-35-41 cluster we only detected mir-37 in the germline ablated animals, but only in 30% of the individual worm samples. The levels of mir-37 we measured were much lower than those observed in wild type young adult animals (Figure 2F and compare Supplemental Tables 1 and 2)."

aging : mir-240 : mir-35 : mir-36 : mir-37 : mir-39 : mir-44 : mir-51

"Analysis of the expression profiles in single worms lead to the identification of miRNAs which had significantly altered levels between young and old animals (Figure 1 and Table 1). Some miRNAs that showed altered levels with age had higher levels later in life. Of these, the most statistically significant increases were observed for miRNAs; mir-51, mir-44, mir-240 and the members of the mir-35-41 cluster (Figure 1B-D and Figure 2A-E). "

mir-241 : mir-48

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