In wild-type, GFP:MEI-1 levels remain steady for w25 30 min after egg exit from the spermatheca. In contrast, in egg-3(tm1191) mutants, GFP:MEI-1 levels started to decline immediately after spermatheca exit, reaching background levels by 30 min.
The recruitment of RAB-7 onto the phagosome was delayed from 70 15 min in WT(n=11) to 102 30 min in epg-5 (n = 12) mutants. The GFP::RAB-7 ring was less stable in the epg-5 mutant than that of WT.
ds RNA transcribed from DNA encoding for F40E10.1 was injected into worms carrying an egl-17::GFP integrated line. The construct did not altered the late stage expression of the reporter construct.
"Nuclear localization induced by starvation is rapidly reversed by exposure to food (E. coli). When starved animals with nuclear localized DAF-16::GFP are placed on an NGM plate seeded with E. coi, delocalization is first evident at 5 min and is complete in 10 min (see Figure 3b4)."
ds RNA transcribed from DNA encoding for K08A8.2 was injected into worms carrying an egl-17::GFP integrated line. The construct did not altered the late stage expression of the reporter construct.
When we examined the effect of removing lin-1 activity on the expression of the full-length reporter arIs131[lag-2p::2Xnls::yfp] or the lag-2p(min) reporter arEx1098[lag-2p(min):: 2nls-yfp], we observed strong and uniform derepression of lag-2 in all six VPCs in lin-1(0) mutants.