Copper [100uM] : hsp-16.2

"When tested with a few representative metals, it was found that copper at 47h LC50[Cu] concentration, i.e. about 100uM, was sufficient to induce the reporter gene [hsp16-2::GFP] expression in 5h. Cadmium had the identical effect at 273uM, which corresponds to LC20[Cd]. Similarly, mercury induced gfp expression at 15uM, i.e. LC20[Hg], under the same assay conditions... Zinc, on the other hand, did not induce gfp expression in KC135 efficiently, and thus the inducible dose could not be determined."

Zinc : hsp-16.2

"When tested with a few representative metals, it was found that copper at 47h LC50[Cu] concentration, i.e. about 100uM, was sufficient to induce the reporter gene [hsp16-2::GFP] expression in 5h. Cadmium had the identical effect at 273uM, which corresponds to LC20[Cd]. Similarly, mercury induced gfp expression at 15uM, i.e. LC20[Hg], under the same assay conditions... Zinc, on the other hand, did not induce gfp expression in KC135 efficiently, and thus the inducible dose could not be determined."

Cadmium [273uM] : hsp-16.2

"When tested with a few representative metals, it was found that copper at 47h LC50[Cu] concentration, i.e. about 100uM, was sufficient to induce the reporter gene [hsp16-2::GFP] expression in 5h. Cadmium had the identical effect at 273uM, which corresponds to LC20[Cd]. Similarly, mercury induced gfp expression at 15uM, i.e. LC20[Hg], under the same assay conditions... Zinc, on the other hand, did not induce gfp expression in KC135 efficiently, and thus the inducible dose could not be determined."

Mercury [15uM] : hsp-16.2

"When tested with a few representative metals, it was found that copper at 47h LC50[Cu] concentration, i.e. about 100uM, was sufficient to induce the reporter gene [hsp16-2::GFP] expression in 5h. Cadmium had the identical effect at 273uM, which corresponds to LC20[Cd]. Similarly, mercury induced gfp expression at 15uM, i.e. LC20[Hg], under the same assay conditions... Zinc, on the other hand, did not induce gfp expression in KC135 efficiently, and thus the inducible dose could not be determined."