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"unc-84(n369) embryos containing between 50 and a few hundred nuclei were stained pair-wise for UNC-84 and either nucleoporins (using mAb414) or each of three other nuclear lamina proteins: Ce-lamin (Liu et al., 2000), Ce-emerin, and Ce-MAN1 (Lee et al., 2000). The nucleoporins and all three lamina proteins remained properly localized to the nuclear envelope in the absence of UNC-84 (Figure 7)."

"unc-84(n369) embryos containing between 50 and a few hundred nuclei were stained pair-wise for UNC-84 and either nucleoporins (using mAb414) or each of three other nuclear lamina proteins: Ce-lamin (Liu et al., 2000), Ce-emerin, and Ce-MAN1 (Lee et al., 2000). The nucleoporins and all three lamina proteins remained properly localized to the nuclear envelope in the absence of UNC-84 (Figure 7)."

"We used indirect immunofluorescence to localize endogenous UNC-84 in lamin-deficient lmn-1(RNAi) embryos, created by injecting double-stranded lamin RNA into the syncytial gonad of adult hermaphrodites (Liu et al., 2000). Embryos with more than 50 cells were triple-stained for DNA, endogenous Celamin, and endogenous UNC-84 (Figure 8). UNC-84 staining at the nuclear envelope was not detectable in lamin-deficient embryos, demonstrating that UNC-84 requires Ce-lamin for stable retention at the nuclear envelope (Figure 8)."

"tlk-1(RNAi) experiments were performed in C. elegans strains harboring transgenes in which the regulatory regions of RNAPII-dependent embryonically expressed genes had been fused to a GFP reporter. When hermaphrodites were fed HT115 bacteria transformed with the RNAi feeding vector L440 without an insert, embryonic GFP expression was readily apparent in the four strains tested (med-1::GFP [28], end-3::GFP [29], elt-2::GFP [30], and myo-2::GFP;pes-10::GFP [M. Edgley, J. Liu, D. Riddle, and A. Fire, personal commjnication]). GFP expression was not detectable in RNAPII/ama-1(RNAi) embryos and was greatly reduced in tlk-1(RNAi) embryos (Figure 3)."

"tlk-1(RNAi) experiments were performed in C. elegans strains harboring transgenes in which the regulatory regions of RNAPII-dependent embryonically expressed genes had been fused to a GFP reporter. When hermaphrodites were fed HT115 bacteria transformed with the RNAi feeding vector L440 without an insert, embryonic GFP expression was readily apparent in the four strains tested (med-1::GFP [28], end-3::GFP [29], elt-2::GFP [30], and myo-2::GFP;pes-10::GFP [M. Edgley, J. Liu, D. Riddle, and A. Fire, personal commjnication]). GFP expression was not detectable in RNAPII/ama-1(RNAi) embryos and was greatly reduced in tlk-1(RNAi) embryos (Figure 3)."

"tlk-1(RNAi) experiments were performed in C. elegans strains harboring transgenes in which the regulatory regions of RNAPII-dependent embryonically expressed genes had been fused to a GFP reporter. When hermaphrodites were fed HT115 bacteria transformed with the RNAi feeding vector L440 without an insert, embryonic GFP expression was readily apparent in the four strains tested (med-1::GFP [28], end-3::GFP [29], elt-2::GFP [30], and myo-2::GFP;pes-10::GFP [M. Edgley, J. Liu, D. Riddle, and A. Fire, personal commjnication]). GFP expression was not detectable in RNAPII/ama-1(RNAi) embryos and was greatly reduced in tlk-1(RNAi) embryos (Figure 3)."

"tlk-1(RNAi) experiments were performed in C. elegans strains harboring transgenes in which the regulatory regions of RNAPII-dependent embryonically expressed genes had been fused to a GFP reporter. When hermaphrodites were fed HT115 bacteria transformed with the RNAi feeding vector L440 without an insert, embryonic GFP expression was readily apparent in the four strains tested (med-1::GFP [28], end-3::GFP [29], elt-2::GFP [30], and myo-2::GFP;pes-10::GFP [M. Edgley, J. Liu, D. Riddle, and A. Fire, personal commjnication]). GFP expression was not detectable in RNAPII/ama-1(RNAi) embryos and was greatly reduced in tlk-1(RNAi) embryos (Figure 3)."

"tlk-1(RNAi) experiments were performed in C. elegans strains harboring transgenes in which the regulatory regions of RNAPII-dependent embryonically expressed genes had been fused to a GFP reporter. When hermaphrodites were fed HT115 bacteria transformed with the RNAi feeding vector L440 without an insert, embryonic GFP expression was readily apparent in the four strains tested (med-1::GFP [28], end-3::GFP [29], elt-2::GFP [30], and myo-2::GFP;pes-10::GFP [M. Edgley, J. Liu, D. Riddle, and A. Fire, personal commjnication]). GFP expression was not detectable in RNAPII/ama-1(RNAi) embryos and was greatly reduced in tlk-1(RNAi) embryos (Figure 3)."

"tlk-1(RNAi) experiments were performed in C. elegans strains harboring transgenes in which the regulatory regions of RNAPII-dependent embryonically expressed genes had been fused to a GFP reporter. When hermaphrodites were fed HT115 bacteria transformed with the RNAi feeding vector L440 without an insert, embryonic GFP expression was readily apparent in the four strains tested (med-1::GFP [28], end-3::GFP [29], elt-2::GFP [30], and myo-2::GFP;pes-10::GFP [M. Edgley, J. Liu, D. Riddle, and A. Fire, personal commjnication]). GFP expression was not detectable in RNAPII/ama-1(RNAi) embryos and was greatly reduced in tlk-1(RNAi) embryos (Figure 3)."