"We used indirect immunofluorescence to localize endogenous UNC-84 in lamin-deficient
lmn-1(RNAi) embryos, created by injecting double-stranded lamin RNA into the syncytial gonad of adult hermaphrodites (Liu et al., 2000). Embryos with more than 50 cells were triple-stained for DNA, endogenous Celamin, and endogenous UNC-84 (Figure 8). UNC-84 staining at the nuclear envelope was not detectable in lamin-deficient embryos, demonstrating that UNC-84 requires Ce-lamin for stable retention at the nuclear envelope (Figure 8)."