Precursor and mature
let-7 accumulated, whereas the SL1 trans-spliced primary transcript was under-represented, in
lin-28 mutants at the 10-h time point. Closer analysis of pri-
let-7 (primary mRNA) levels during the late L1 and early L2 stages revealed significantly reduced levels of total pri-
let-7 during the initial peak of expression at 10 h in
lin-28(
n719) compared to WT worms.