tat-1 RNAi alters phosphatidylserine (PS) distribution in the membrane of C. elegans germ cells so that PS staining was observed on the surface of many normal germ cells. In wild-type C. elegans, no PS staining was observed on the surface of normal germ cells. Similar amounts of germ cell PS staining were observed in
scrm-4(
tm624) scrm-(
tm650),
scrm-3(
tm631) triple mutant animals treated with
tat-1(RNAi) to that seen in wild-type animals treated with
tat-1(RNAi). Thus, loss of phospholipid scramblases does not affect PS externalization in
tat-1 deficient animals.