lem-2 : unc-84

"unc-84(n369) embryos containing between 50 and a few hundred nuclei were stained pair-wise for UNC-84 and either nucleoporins (using mAb414) or each of three other nuclear lamina proteins: Ce-lamin (Liu et al., 2000), Ce-emerin, and Ce-MAN1 (Lee et al., 2000). The nucleoporins and all three lamina proteins remained properly localized to the nuclear envelope in the absence of UNC-84 (Figure 7)."

emr-1 : unc-84

"unc-84(n369) embryos containing between 50 and a few hundred nuclei were stained pair-wise for UNC-84 and either nucleoporins (using mAb414) or each of three other nuclear lamina proteins: Ce-lamin (Liu et al., 2000), Ce-emerin, and Ce-MAN1 (Lee et al., 2000). The nucleoporins and all three lamina proteins remained properly localized to the nuclear envelope in the absence of UNC-84 (Figure 7)."

ain-2 : gld-1

36 other proteins were identified in the AIN-2::GFP IP sample by at least two different peptides but were absent from the control GFP IP sample. GLD-1 and ACO-1, two RNA-binding proteins involved in translational inhibition (Gourley et al., 2003; Jan et al., 1999; Lee and Schedl, 2001), were also found to be in the AIN-2 IP sample, suggesting that miRNA may collaborate with RNA-binding proteins to repress the translation of target mRNAs.

aco-1 : ain-2

36 other proteins were identified in the AIN-2::GFP IP sample by at least two different peptides but were absent from the control GFP IP sample. GLD-1 and ACO-1, two RNA-binding proteins involved in translational inhibition (Gourley et al., 2003; Jan et al., 1999; Lee and Schedl, 2001), were also found to be in the AIN-2 IP sample, suggesting that miRNA may collaborate with RNA-binding proteins to repress the translation of target mRNAs.

ain-2 : tsn-1

36 other proteins were identified in the AIN-2::GFP IP sample by at least two different peptides but were absent from the control GFP IP sample. TSN-1 is a homolog of micrococcal nuclease that was shown to be associated with RISC and required for let-7 miRNA function (Caudy et al., 2003), which is consistent with the involvement of AIN-2 in the miRNA effector complex. GLD-1 and ACO-1, two RNA-binding proteins involved in translational inhibition (Gourley et al., 2003; Jan et al., 1999; Lee and Schedl, 2001), were also found to be in the AIN-2 IP sample, suggesting that miRNA may collaborate with RNA-binding proteins to repress the translation of target mRNAs.